Hvozdiuk Ya. V., Seliuta A. A., Poliakova H. L., Gurina T. M.

USE OF POLYVINYL ALCOHOL AS A COMPONENT OF CRYOPRESERVATIVE SOLUTION TO CRYOPRESERVE HUMAN ERYTHROCYTES

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Hvozdiuk Ya. V., Seliuta A. A., Poliakova H. L., Gurina T. M.

Heading:

BIOLOGY

Type of article:

Scientific article

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Cryopreservation of erythrocytes is the main method for long-term storage of blood cell components, but the ef fectiveness of this process is limited by damage caused by the formation and recrystallization of ice crystals. A prom ising approach to reducing cryodamage is the use of macromolecular impermeable cryoprotectants, in particular polyvinyl alcohol (PVA). The aim of the study was to investigate the effect of PVA with a molecular weight of 9 kDa at different concentrations on ice recrystallization processes and to evaluate its effectiveness in cryopreserving human erythrocytes. The study's objective was to examine human erythrocytes obtained from donor blood. PVA solutions in phosphate-saline buffer at concentrations ranging from 0.1 to 1.5% were used. Phase transformations and ice recrystallization were studied using thermomechanical analysis. The cryopreservation modes used differed in cooling rates and had the same heating rate. The preservation of human erythrocytes was assessed by hemolysis levels. It was found that an increase in PVA concentration contributes to the effective inhibition of ice recrystallization, and at a concentration of about 1%, this process is almost completely suppressed. It was shown that uncontrolled cooling leads to complete erythrocyte hemolysis. The best cell preservation was achieved with slow, controlled cooling at 3 degrees/min in a 1% PVA solution. Polyvinyl alcohol exhibits cryoprotective activity and is a promising component of cryopreservation media for human erythrocytes under slow, controlled cooling conditions.

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Bibliography:

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Publication of the article:

«Bulletin of problems biology and medicine», 2026 Issue 1, 180, 171-181 pages, index UDC 678.744.7:612.111.014.43:57.086.13

DOI:

10.29254/2077-4214-2026-1-180-171-181