Chebernina I. O., Nebesna Z. M.

ULTRASTRUCTURAL CHANGES OF HIPPOCAMPAL HEMOCAPILLARIES IN RATS WITH INDUCED COLORECTAL CANCER AND UNDER CORRECTION WITH NANOMATERIALS

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About the author:

Chebernina I. O., Nebesna Z. M.

Heading:

MORPHOLOGY

Type of article:

Scientific article

Annotation:

The article presents an electron microscopic study of changes in the microcirculatory bed of the hippocampus in white rats under conditions of induced colon adenocarcinoma and following correction with Au/Ag/Fe nanoma terials. The obtained results made it possible to assess the nature of hemocapillary alterations during a systemic pathological process as well as the protective effect of nanoparticles. It was determined that prolonged exposure to the carcinogen N,N-dimethylhydrazine was accompanied by pronounced destructive changes in the hemocapillaries of the hippocampus. These changes manifested as uneven blood filling, stasis and erythrocyte sludge, narrowing or collapse of the vascular lumen, endothelial cell swelling, a decrease in the number of micropinocytotic vesicles, dis ruption of intercellular contacts, and deformation of endothelial cell nuclei. The observed alterations indicate the de velopment of endothelial dysfunction, impaired microcirculation, and disturbances in transcapillary water exchange, which are manifestations of the response to systemic inflammation and oxidative stress typical of DMH-induced carcinogenesis. Following the administration of the Au/Ag/Fe nanoparticle composition, a partial restoration of the ultrastructure of hippocampal hemocapillaries was observed. A reduction in perivascular edema, more uniform blood filling of vessels, restoration of capillary lumens, and a decreased frequency of stasis were noted. In endothe lial cells, cytoplasmic swelling decreased, the number of micropinocytotic vesicles increased, organelles acquired a more orderly arrangement, and euchromatin predominated in the nuclei, indicating partial normalization of cellular metabolic activity. Greater preservation of mitochondria and stabilization of the basement membrane structure were also observed. Thus, the obtained electron microscopic data of the hippocampus may indicate the endothelioprotective and microcirculatory effects of gold, silver, and iron nanoparticles, contributing to the restoration of the ultrastructural organization of hippocampal hemocapillaries under conditions of DMH-induced colon carcinogenesis. These findings confirm the potential of nanomaterials to reduce the degree of vascular alterations in the hippocampus and may positively influence the structural and functional stability of nervous tissue.

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Bibliography:

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Publication of the article:

«Bulletin of problems biology and medicine», 2026 Issue 1, 180, 460-467 pages, index UDC 10.616.345-006.6:611.8:612.13:620.3

DOI:

29254/2077-4214-2026-1-180-460-467