Solovey Y. M., Polevoy V. P., Solovey M. M.

METHODS AND METHODOLOGIES

Scentific article

. In the experiment, abdominal sepsis of varying severity was modeled on 273 nonlinear white rats: mild, moderate and severe course. Intact animals served as a control group. Internal organs were taken and histological sections of the spleen were prepared at 12, 48 and 78 hours of the experiment. Investigated the structure of the polycrystalline component of biological preparations of histological sections of the spleen differential diagnosis of the severity of septic condition by digital Stokes polarimetric fluorescence microscopy Experimentally tested the optical arrangement of the Stokes polarimetric mapping system with vector-parametric analysis of fluorescence microscopic images of histological sections of the spleen of laboratory rats. An album of vector-parametric polarization maps and distributions of the number of characteristic values of the crystallization parameter at thepoints of digital fluorescence microscopic images of histological sections of the spleen from control group 1 and experimental groups 2 – 4 with different severity of septic pathology was obtained. The statistical reliability of differentiation of vector-parametric maps of fluorescent microscopic images of histological sections of the spleen from control group 1 and experimental groups 2 – 4 with different severity of septic pathology was determined. The most diagnostically sensitive statistical criteria for the differentiation of vector-parametric polarization maps of fluorescence microscopic images of histological sections of the spleen of rats from control group 1 and experimental groups 2 – 4 with different severity of septic pathology were found. The operational characteristics of the diagnostic power of the method of Stokes polarimetric microscopy of fluorescence microscopic images of histological sections of the spleen of rats of control and experimental groups were determined. The balanced accuracy of: differentiation of healthy and septic rats; intergroup differentiation of the severity of the septic process of sick rats; intragroup differentiation of septic rats was established.

abdominal sepsis,spleen,fluorescence microscopy,Stokes vector

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«Bulletin of problems biology and medicine» Issue 4 (167), 2022 year, 269-280 pages, index UDK 617.55-002:616.94]-07:612.411.086.2

10.29254/2077-4214-2022-4-167-269-280