Garmash O. V., Gubina-Vakulik G. I.

DENTISTRY

Scentific article

This research is an experimental study of the long-term effect of fetal macrosomia on the morphofunctional state of pulp, hard tooth tissues, and periodontal tissues of three-month- and six-month-old animals. The investigation takes into account the particularities of animal intrauterine somatotype: macrosomia with acceleration, macrosomia with harmonious (well-balanced) intrauterine development, and relative intrauterine insufficiency of body weight or macrosomia with intrauterine obesity. Object and research methods. This study has been conducted using rats of the Wistar Albino Glaxo population. The offspring of such rats were removed from the experiment on 90th and 180th day after birth. Macrosomia was modeled using four different macrosomia formation models. Immediately after birth, the rats were weighed and divided into five groups (each of 5–6 individuals) taking into account the calculated weight-height parameters and the ways of modeling macrosomia. The carious process intensity was calculated, 5 – 6 μm thick sections were stained with hematoxylin and eosin, Halocyanin-chromium alum according to Einarson (for total nucleic acids), picrofuxin according to Van Gieson (to determine interstitial connective tissue collagen), and the PAS-reaction was performed on glycoproteins. Conclusions. The state of hard tooth tissues and tooth pulp is reliably worse in animals born with the signs of intrauterine obesity. Whereas a negative effect has been revealed on periodontal tissue when the prenatal period proceeded with relative insufficiency of body weight or the prenatal period was characterized by adequately increased somatometric indices of body weight and body length, and the animals were borne with macrosomia. Correlation analysis showed that the larger the body mass index of an animal at birth, the higher its tendency to carious lesions formation.

fetal macrosomia, rat, oral cavity.

1. Garmash OV. Osoblyvosti stomatolohichnoho statusu ditei, yaki narodylysia z makrosomiieiu, u period tymchasovoho prykusu. Visnyk problem biolohii i medytsyny. 2018;4(1/146):246-53. [in Ukrainian].
2. Garmash O. Dependence of Deciduous Tooth Eruption Terms and Tooth Growth Rate on the Weight-Height Index at Birth in Macrosomic Children over the First Year of Life. Acta Medica (Hradec Králové). 2019;62(2):62-8.
3. Garmash OV. Oral health abnormalities in children born with macrosomia established during mixed dentition period. Wiad Lek. 2019;72(5.1):823-31.
4. Morikawa M, Cho K, Yamada T, Yamada T, Sato S, Minakami H. Fetal macrosomia in Japanese women. J Obstet Gynaecol Res. 2013 May;39(5):960-5. DOI: 10.1111/j.1447-0756.2012.02059.x
5. Sheshukova OV, Veretilnyk AV. Vplyv hihiienichnykh ta kharchovykh zvychok na urazhenist kariiesom ditei molodshoho shkilnoho viku z normalnoiu ta nadmirnoiu masoiu tila. Aktualni problemy suchasnoi medytsyny: Visnyk Ukrainskoi medychnoi stomatolohichnoi akademii. 2019;19(4/68):77-82. [in Ukrainian].
6. Garmash O, Gubina-Vakulik G, Vondrášek D. Three dimensional image analysis of minor salivary glands in 180-day rats born with macrosomia. Med J (Krag). 2018;52(1):7-14. DOI: 10.5937/mckg52-16885
7. Garmash ОV, Gubina-Vakulyk GІ. Morfofunktsionalnyi stan pryvushnykh slynnykh zaloz trymisiachnykh shchuriv pry eksperymentalno modelovanii vnutrishnoutrobnii makrosomii. Pathologia. 2018;15(1):81-7. DOI: 10.14739/2310-1237. 2018.1.129324 [in Ukrainian].
8. Pylypiuk OY. Obhruntuvannia kompleksnoi profilaktyky i likuvannia kariiesu zubiv u ditei z yuvenilnim revmatoidnym artrytom [avtoreferat]. Uzhhorod: Derzh. VNZ «Uzhhorod. nats. un-t»; 2019. 20 s. [in Ukrainian].
9. Pearse AGE. Histochemistry: Theoretical and Applied. Fourth edition. Churchill Livingstone: Edinburgh; 1985. 624 p.
10. Merkulov GA. Kurs patogistologicheskoj tekhniki. Moskva; 1969. 425 s. [in Russian].
11. Sarkisov DS, Perov YuL. Mikroskopicheskaya tekhnika: rukovodstvo dlya vrachej i laborantov. Moskva: Medicina; 1996. 427 s. [in Russian].
12. Andreollo NA, Santos EF, Araújo MR, Lopes LR. Rat’s age versus human’s age: what is the relationship? Arq Bras Cir Dig. 2012;25(1):49-51. DOI: 10.1590/S0102-67202012000100011
13. Habeeb Bin Mohsin, Al Barshaik S. Epigenetics in Dentistry: A Literature Review. Journal of Clinical Epigenetics. 2017 January;3(1):1-4.
14. Baranova MS, Gubina-Vakyulyk GI, Gorbach TV, Tkachenko AS. Metabolic and histological features of dental tissue in adult rat offspring in maternal hypokinesia. Comp Clin Pathol. 2017;26:1329-34.
15. Baranova MS, Gubina-Vakulik GI. Vliyaniye materinskoy gipokinezii pri vynashivanii potomstva na morfofunktsionalnoye sostoyaniye tkaney zubov vzroslykh molodykh potomkov. Vіsnik problem bіologіі і meditsini. 2015;4(2):339-43. [in Russian].
16. Zhulev EN, Kochubeynik AV, Lapshin RD. Eksperimentalnoye modelirovaniye vospalitelnykh zabolevaniy parodonta. Fundamentalnyye issledovaniya. 2015;1(4):744-7. [in Russian].
17. Borovskiy EV. Terapevticheskaya stomatologiya. M.: Meditsinskoye informatsionnoye agentstvo; 2011. 840 s. [in Russian].
18. Gemonov VV, Lavrova EN, Falin LI. Gistologiya i embriologiya organov polosti rta i zubov: ucheb. Posobiye. M.: GEOTAR-Media; 2016. 312 s.: il. [in Russian].

«Bulletin of problems biology and medicine» Issue 4 Part 2 (154), 2019 year, 365-372 pages, index UDK 616.314-092.9-007.61

10.29254/2077-4214-2019-4-2-154-365-372