Yevchuk Yu. I.

THE EFFECTIVENESS OF USING A COMBINED FIBRIN-BONE SCAFFOLD FOR BONE TISSUE REPAIR IN JAW DEFECTS

---

Show/Download

About the author:

Yevchuk Yu. I.

Heading:

DENTISTRY

Type of article:

Scientific article

Annotation:

The article discusses the effectiveness of the developed new methodology for improvement of the efficiency of bone augmentation in the replacement of bone tissue defects. The objective of the study was to enhance the efficacy of bone defect restoration of the jaws with allo-autologous material obtained by means of simultaneous centrifuga- tion of granules of demineralised bone-plastic material and patient’s venous blood. For the clinical trials, a total of 50 patients aged between 20 and 40 years diagnosed with radicular cysts of the jaws, were selected. All patients were divided into two groups – group I and II, who were performed cystectomy and bone grafting, respectively. Scaffold was prepared in accordance with the protocol developed by the present authors and used in group I. The results of the aforementioned postoperative radiological examinations confirm the effectiveness of the filter and the technique developed by the present authors, which allows for the creation of a stable fibrin matrix with embedded granules of bone-plastic material. This effectiveness was reflected in the simultaneous uniform regeneration of bone tissue during the sixth month over the entire thickness of the defect in group I in 21 (84%) patients. This is evidenced by the organised architectonics of bone tissue with a pronounced trabecular pattern, which correlated with bone density in- dices ranging from 780.69+18.77 to 516.31+31.19 HU. In this group of patients, these indices corresponded to intact bone. Conversely, in group II, only 7 patients (28%) exhibited complete bone restoration. The clinical and radiological analysis performed demonstrated the efficacy of the technique developed by us, as indicated by the positive results in the form of complete bone restoration in 84% of patients in group I and only in 28% of patients in group II.

Tags:

Bibliography:

1. Korniyenko MM. Vedennya rannoho pislyaoperatsiynoho periodu pislya tsystektomiyi iz zastosuvannyam preparatu na osnovi benzydaminu hidrokhlorydu. Novyny stomatolohiyi. 2015;2:45-8. [in Ukrainian].
2. Lytvynets-Holutyak UYe. Doslidzhennya makro- ta mikroelementnoho statusu u khvorykh na odontohenni kysty. Aktualni problemy suchasnoyi medytsyny: Visnyk Ukrayinskoyi medychnoyi stomatolohichnoyi akademiyi. 2013;3(43):47-50. [in Ukrainian].
3. Ruzin GP, Tkachenko OV. Klinicheskiye proyavleniya toksicheskogo osteomiyelita v zavisimosti ot davnosti upotrebleniya narkotika. Ukrainskiy stomatologíchniy almanakh. 2015;1:47-52.
4. Avetikov DS, Stavytskyy SA, Lokes KP, Yatsenko IV. Otsinka efektyvnosti auhmentatsiyi alveolyarnoho hrebnya na etapi pidhotovky do dentalnoyi implantatsiyi. Visnyk problem biolohiyi i medytsyny. 2016;1(131):240-2. [in Ukrainian].
5. Pavlenko AV, Dmitriyeva EA, Luzin VI. Gistologicheskoye stroyeniye regenerata pri zapolnenii kostnogo defekta materialami easygraft i trikaltsiyfosfatom. Morfologiya. 2011;5(2):49-54.
6. Pavlenko OV, Dmytriyeva YeO. Morfolohichni osnovy vyboru kistkovoplastychnykh materialiv u parodontolohiyi. Morfolohiya. 2011;5(1):5- 12. [in Ukrainian].
7. Berwig KH, Baldasso C, Dettmer A. Production and characterization of poly(3-hydroxybutyrate) generated by Alcaligenes latus using lactose and whey after acid protein precipitation process. Bioresour. Technol. 2016;218:31-7.
8. Hapach LA, VanderBurgh JA, Miller JP, Reinhart-King CA. Manipulation of in vitro collagen matrix architecture for scaffolds of improved physiological relevance. Physical Biology. 2015;12(6):061002. DOI: 10.1088/1478-3975/12/6/061002.
9. Pantus AV, Rozhko MM, Paliychuk VI, Kovalchuk NY, Melnyk NS. Microstructure of biopolymer micro-fibrous scaffold and its influence on the ability to retain medicines and tissue regeneration. Georgian medical news. 2023;3(336):37-44.
10. Crisci A, Lombardi D, Serra E, Lombardi G, Cardillo F, Crisci M. Standardized protocol proposed for clinical use of L-PRF and the use of L-PRF Wound Box®. J Unexplored Med Data. 2017;2:77-87. DOI: 10.20517/2572-8180.2017.17.
11. Kubesch A, Barbeck M, Al-Maawi S, Orlowska A, Booms P, Sader R, et al. A low-speed centrifugation concept leads to cell accumulation and vascularization of solid platelet-rich fibrin: An experimental study in vivo. Platelets. 2019;30(3):329-340. DOI:10.1080/09537104.2018.1445835.
12. Lee HM, Shen EC, Shen JT, Fu E, Chiu HC, Hsia YJ. Tensile strength, growth factor content and proliferation activities for two platelet concentrates of platelet-rich fibrin and concentrated growth factor. J. Dent. Sci. 2020;15(2):141-146. DOI: 10.1016/j.jds.2020.03.011.
13. Li W, Sigley J, Pieters M, Helms C, Nagaswami C, Weisel JW, et al. Fibrin Fiber Stiffness Is Strongly Affected by Fiber Diameter, but Not by Fibrinogen Glycation. Biophys. J. 2016;110(6):1400-1410. DOI: 10.1016/j.bpj.2016.02. 021.
14. McLellan J, Plevin S. Temporal release of growth factors from platelet- rich fibrin (PRF) and platelet-rich plasma (PRP) in the horse: a comparative in vitro analysis. International Journal of Applied Research in Veterinary Medicine. 2014;12(1):48-57.
15. Caymaz G, Uyanik O. Comparison of the effect of advanced platelet-rich fibrin and leukocyte- and platelet-rich fibrin on outcomes after removal of impacted mandibular third molar: A randomized split-mouth study. Niger. J. Clin. Pract. 2019;22(4):546-552. DOI: 10.4103/njcp.njcp_473_18.
16. Canellas JVDS, Medeiros PJD, Figueredo CMDS, Fischer RG, Ritto FG. Platelet-richfibrin in oral surgical procedures: A systematic review and meta-analysis. Int. J. Oral Maxillofac. Surg. 2019;48(3):395-414. DOI: 10.1016/j.ijom.2018.07.007.
17. Caruana A, Savina D, Macedo JP, Soares SC. From Platelet- Rich Plasma to Advanced Platelet-Rich Fibrin: Biological Achievements and Clinical Advances in Modern Surgery. Eur. J. Dent. 2019;13(2):280-286. DOI: 10.1055/s-0039-1696585.
18. Crisci A, Manfredi S, Crisci M. Fibrinrich in Leukocyte- Platelets (L-PRF) and Injectable Fibrin Rich Platelets (I-PRF), two opportunity in regenerative surgery: Review of the sciences and literature. IOSR Journal of Dental and Medical Sciences (IOSR-JDMS). 2019;18:66-79.
19. Choukroun J, Ghanaati S. Reduction of relative centrifugation force within injectable platelet-rich-fibrin (PRF) concentrates advances patients’ own inflammatory cells, platelets and growth factors: The first introduction to the low speed centrifugation concept. Eur. J. Trauma Emerg. Surg. 2018;44(1):87-95. DOI: 10.1007/s00068-017-0767-9.
20. Siawasch SAM, Andrade C, Castro AB, Teughels W, Temmerman A, Quirynen M. Impact of local and systemic antimicrobials on leukocyte- and platelet rich fibrin: an in vitro study. Scientific Reports. 2022;12(1):2710. DOI: 10.1038/s41598-022-06473-4.
21. Crisci A, Barillaro MC, Lepore G, Cardillo F. L-PRF Membrane (FibrinRich in Platelets and Leukocytes) and Its Derivatives (A-PRF, i- PRF) are Help ful as a Basis of Stem Cells in Regenerative Injury Treatment: Trial Work on the Horse. International Blood Research & Reviews. 2019;10(2):1-14.
22. Crisci A, Benincasa G, Crisci M, Crisci F. Leukocyte Platelet-Rich Fibrin (L-PRF), a new biomembrane useful in tissue repair: basic science and literature review. Biointerface Research in Applied Chemistry. 2018;8(5):3635-43.
23. Kim BJ, Kwon TK, Baek HS, Hwang DS, Kim CH, Chung IK, et al. A comparative study of the effectiveness of sinus bone grafting with recombinant human bone morphogenetic protein 2-coated tricalcium phosphate and platelet-rich fibrin-mixed tricalcium phosphate in rabbits. Oral Surg Oral Med Oral Pathol Oral Radiol. 2012;113(5):583-92. DOI:10.1016/j.tripleo.2011.04.029.
24. De Almeida NCPM, Dos Santos NBM, Completo AMG, De Oliveira FGV. Tensile strength assay comparing the resistance between two different autologous platelet concentrates (leucocyte-platelet rich fibrin versus advanced- plateletrichfibrin): A pilotstudy. Int. J. Implant Dent. 2021;7(1):1. DOI: 10.1186/s40729-020-00284-w.
25. El Bagdadi K, Kubesch A, Yu X, Al-Maawi S, Orlowska A, Dias A, et al. Reduction of relative centrifugal forces increases growth factor release within solid platelet- rich fibrin (PRF)-based matrices: a proof of concept of LSCC (low speed centrifugation concept). European Journal of Trauma and Emergency Surgery. 2019;45(3):467-79.

Publication of the article:

«Bulletin of problems biology and medicine», Issue 2,177, 490-505 pages, index UDC 616.314-089.844+616.716

DOI:

10.29254/2077-4214-2025-2-177-490-505