The aim of this research was to study the effect of a new group of epoxide-containing polyoxypropyl- ene polyols on indices of immunobiological reactivity assessment in prolonged exposure to subtoxic doses. The research was performed with the employment of stage schedule in order to determine allergic effect exert- ed by laproxides. The first stage implied investigation of sensitizing properties possessed by the compounds under study, namely polyoxypropylene triol triglycidyl ether M. m. 700 (L-703), M. m. 500 (L-503), М. m. 300 (L-303). The trial was performed on guinea pigs with intradermal, patch and conjunctival tests. The second stage involved the establishment of threshold doses in subacute trial on white Wistar rats. The ani- mals were administered the substances through a metallic tube perorally in the dose of 1/100, 1/1000 and 1/10000 LD50 for 45 days on a daily basis along with application of three immunologic tests: specific leukocyte lysis reaction, specific basophil damage reaction and specific leukocyte agglomeration reaction according to common methods. The trial involved investigation of plasmocytic reaction of the spleen and lymphatic nodes. Joint interaction of cellular and humoral immunity was studied on hybrid mice (СВА∙С57ВL)∙F1, BALB/C, CBA/ Lac in compliance with guidelines concerning the effect of ambient and occupational environment on immunobio- logical reactivity. Following the completion of the subacute trial the mice were immunized by mutton erythrocytes. On the 4 – 6th day following the administration of Т-dependent antigen the spleen was removed to determine the spleen index, the total number of nucleus-containing cells in the spleen and the number of nucleus-containing cells per milligram of organ tissue, the number of rosette-forming cells, lymphocyte transformation reaction in re- sponse to cellular stimulus such as phytohemagglutinin, liposaccharides, specific allergen, neutrophil damage index, hemolysin-producing function of nucleus-containing cells, antibody-forming and antigen-binding function of immune competent cells. Homotransplantation activity of lymph node cells was assessed by the level of allogenic endocolony-formation inhibition in mice BALB/C. Functional activity of Т- and В-lymphocytes was determined by the index of lymph node cells and splenocytes stimulation by phytohemagglutinin and liposaccharide mitogens in transformation test. Laproxides were found to be capable of sensitizing the body, conditioning the development of allergic reac- tions accompanied by a significant increase in specific leukocyte agglomeration reaction, specific leukocyte lysis reaction and specific basophil damage reaction. In subtoxic exposure they induce immunologic reconstruction of macrophage-plasmocytic transformation of lymphoid tissue, characterized by an increase in mature cells number and total plasmocytic cells number in lymph nodes and spleen. Laproxides L-303, L-503, L-703 in the dose of 1/100 LD50 inhibit antibody formation, antigen-binding capability of immune-competent cells as well as their homotrans- plantations activity, suppress allogenic endocolony-formation and disturb joint interrelation of cellular and humoral immunity, conditioning the development of immune deficiency. In the dose of 1/1000 LD50 the substances were not found to exert an effect on cellular and humoral immunity indices.
laproxides, xenobiotics, sensibilization, immunological deficiency
«Bulletin of problems biology and medicine» Issue 4 part 1 (113), 2014 year, 150-155 pages, index UDK 612. 017. 1:616-099-008. 9-092. 8:547. 395
