Holovatskyi A. S., Herbut A. O., Kochmar M. Y., Hetsko O. I., Palapa V. Y.

MORPHOLOGY

Scentific article

The density of small, medium and large lymphocytes, plasmocytes and macrophages in the mantle zone of the spleen lymphoid nodes in norm and within a month after the introduction of normal human immunoglobulin antigen stimulation were studied at the histological preparations of the spleen. The first changes in the density of these cells are observed three days after the action of the antigen. At the same time, the largest is the increasing of large lymphocytes number (almost three times), the density of small lymphocytes increases by 1.4%, macrophage density and plasmacytes do not change. The maximum values of the density of immunocompetent cells reaches seven days after the introduction of the antigen. Thus, the number of small lymphocytes is 20.57±0.11, which is 12% more than at the intact group of animals, the density of middle lymphocytes increases by 10%, and the indicators of large lymphocytes are 0.31±0.08, which is 7 times for an intact group of rats. During this period, the density of plastids and macrophages increases and is, accordingly, 0.10±0.06, 0.14±0.06. Enhance of lymphocyte plasmation is observed with electronical microscopic examination. These cells are a large nucleus without a plasmacy-specific pattern, there are also a lot of ribosomes in the cytoplasm. In macrophages, the number and length of cytoplasmic processes increases, and the cytoplasm is saturated with hemosiderinous corpuscles. Summing up the results of this study, we can conclude: 1. One-time antigenic stimulation of the body with “Human Immunoglobulin Normal” causes periodic phase changes in the mantle zone of the lymphoid nodules of the white rats-males spleen in reproductive age, indicating the functional activity of the spleen as a secondary organ of the immune system. 2. Introduction of the antigen causes phase changes in the density of immunocompetent cells with a maximum of seven days after the action of the antigen. 3. Seven days after antigenic stimulation, plasmation of lymphocytes increases and a number of functionally active macrophages grows.

spleen, white rats, lymphocites, antigenic stimulation

1. Herbut AO, Holovatskyi AS, Kochmar MYu, Hetsko OI. Morfofunktsionalna kharakterystyka periarterialnykh limfoidnykh pikhv selezinky bilykh shchuriv-samtsiv riznykh vikovykh hrup u normi. Naukovyi visnyk Uzhhorodskoho universytetu, seriia ″Medytsyna″. 2008;34:12-5. [in Ukrainian].
2. Herbut AO, Kochmar MIu, Holovatskyi AS, Palapa VI. Kharakterystyka shchilnosti klitynnykh elementiv periarterialnykh limfoidnykh pikhv selezinky bilykh shchuriv-samtsiv reproduktyvnoho viku cherez sim dib pislia antyhennoi stymuliatsii orhanizmu. Prykladni aspekty morfolohii. 2009;160-1. [in Ukrainian].
3. Herbut AO, Kochmar MIu, Hetsko OI. Morfofunktsionalna kharakterystyka svitlykh tsentriv limfoidnykh vuzlykiv biloi pulpy selezinky shchurivsamtsiv riznykh vikovykh hrup u normi. Visnyk morfolohii. 2010;16(2):297-0. [in Ukrainian].
4. Klein E. The Anatomy of the Lymphatic System. Edward Klein. BiblioBazaar, LLC; 2008. 156 р.
5. Bibik EYu. Ultrastruktura podmyishechnyih limfaticheskih uzlov intaktnyih polovozrelyih kryis. Ukrainskyi morfolohichnyi almanakh. 2007;3:181. [in Russiаn].
6. Herbut AO, Hetsko OI, Kochmar MIu. Dynamika zmin shchilnosti klitynnykh elementiv periarterialnykh limfoidnykh pikhv selezinky bilykh shchuriv-samtsiv reproduktyvnoho viku protiahom misiatsia pislia antyhennoi stymuliatsii orhanizmu. Trudyi Kryimskogo gosudarstvennogo meditsinskogo universiteta im. S.I. Georgievskogo. 2010;146(5):59-2. [in Ukrainian].
7. Cherkasov EV. Ultrastruktura dendrytnykh klityn tymusa pry eksperymentalnii opikovii khvorobi u shchuriv ta za umov yii likuvannia kombinovanymy hiperosmoliarnymy rozchynamy. Visnyk morfolohii. 2012;18(1):6-10. [in Ukrainian].

«Bulletin of problems biology and medicine» Issue 3 (145), 2018 year, 275-278 pages, index UDK 611.41.42:616-097

10.29254/2077-4214-2018-3-145-275-278