Vislohuzova T. V., Kuliesh D. V., Rozhnova R. A., Galatenko N. A., Narazhayko L. F.

NANOTECHNOLOGY

Scentific article

Modern medicine needs new polymer materials for the manufacture of catheters, drains and a variety of film coatings with resistant antimicrobial properties that will ensure suppression of infection in the product and the focus of inflammation. Since the main requirements for medical polymer materials are biocompatibility and non-toxicity there is a need for preclinical tests. The aim of the work is to study the cytotoxicity and biocompatibility of composite materials filled with silver-containing silica nanocomposite 02AgCu. Object and methods of research. The objects of the study were composite materials based on polyurethaneureas (PUU) with copolymer fragments of poly(vinyl butyral-vinyl acetate-vinyl alcohol) and macrochain extender 1,6-hexamethylenediamine in the structure filled with silver-containing silica nanocomposite 02AgCu in the amount of 1 wt. %. Cytotoxicity studies were performed by tissue culture method on the culture of tissue of subcutaneous fatty tissue of white laboratory rats in vitro. Biocompatibility studies were performed by histological tests by studying cellular responses to the implantation of polymer materials in vivo. Results. According to the results of tissue culture studies it can be concluded that there is no histotoxic effect of the investigated PUU and composite materials on their basis filled with silver-containing silica nanocomposite on cultured cells. Histological tests showed that the polymer samples had a fairly high degree of biocompatibility. Conclusions. The investigated composite materials filled with silver-containing silica nanocomposite 02AgCu in the amount of 1 wt. % are safe and can be proposed for use in various branches of medicine.

composite materials,silver-containing silica nanocomposite,tissue culture,implantation,biocompatibility

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«Bulletin of problems biology and medicine» Issue 3 (166), 2022 year, 460-471 pages, index UDK 678.664:615.28

10.29254/2077-4214-2022-3-166-460-471