Yevchuk Yu. I., Pantus A. V., Kutsyk R. V., Rozhko M. M., Yarmoshuk I. R., Buherchuk O. V., Kovalchuk N. Ye.

STUDY OF ANTIMICROBIAL ACTIVITY AND DISTRIBUTION OF DIFFERENT GROUPS OF ANTIBIOTICS IN BLOOD PLASMA AND THE FIBRIN SCAFFOLD OBTAINED BY US

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Yevchuk Yu. I., Pantus A. V., Kutsyk R. V., Rozhko M. M., Yarmoshuk I. R., Buherchuk O. V., Kovalchuk N. Ye.

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CLINICAL AND EXPERIMENTAL MEDICINE

Type of article:

Scientific article

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The article examines the efficiency of retention and distribution of different groups of antibiotics in a fibrin clot and blood plasma, as well as their effect on the concentration of growth factors. The aim of the study was to in vestigate the features of antibiotic concentration distribution in different blood fractions and in the fibrin matrix, and to identify the possible effect of their concentrations on the mechanical integrity of such a scaffold and on the concentration of growth factors within it. To evaluate the concentration of the antibiotic in fibrin scaffolds, its distri bution in different phases of the experimental system was analyzed. Serum obtained 15 minutes after centrifugation was analyzed, as well as serum isolated after compression of the fibrin clot and the eluate of the compressed fibrin clot. The results of the study showed that, in contrast to cefazolin, lincomycin much more actively saturated the fibrin-platelet clot. Its uniform distribution among all elements of the experimental system was observed only at the minimal saturation dose (0.2 ml of lincomycin solution 300 mg/ml / 7.8 ml of blood). When higher doses of lincomy cin (0.4-0.8 ml) were used for saturation, its active incorporation into the fibrin matrix was observed. Ciprofloxacin, in contrast, demonstrated a lack of tendency to accumulate in the fibrin scaffold, as evidenced by a 4-16-fold decrease in its concentrations in the eluates of compressed clots compared with the corresponding samples of pressed serum. Comparative analysis of lincomycin, cefazolin, and ciprofloxacin showed a significant decrease in the concentration of VEGF-A to 43.37±7.44 pg/ml and a decrease in the concentration of PDGF-AA to 80.57±5.41 pg/ml specifically when cefazolin was added. Thus, the concentrations of antibiotics used in this study are sufficient to ensure an anti microbial effect, provided that the microorganisms do not exhibit resistance to these agents.

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Bibliography:

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Publication of the article:

«Bulletin of problems biology and medicine», 2026 Issue 1, 180, 222-238 pages, index UDC 615.28+615.33+616.15+616.314-089

DOI:

10.29254/2077-4214-2026-1-180-222-238