Gromova А. М., Mityunina N. I., Krutikova E. I.

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

Activation of peroxidation is known to be the key factor in damage of membrane structure in many diseases (“free radical pathology”). One of them can be premature rapture of fetal membranes in parturient women as biologic membranes due to disorder of their structure, reasons of which are often unknown for obstetricians. Pregnancy and childbirth course is accompanied by significant changes in the metabolism of prostaglandins in the body of mother and fetus, influencing the intensity of peroxide oxidation and depend on the latter. However, the main source and spot of prostaglandins’ metabolism during pregnancy are placenta and fetal membranes. Pros­ taglandins weaken the strength of fetal membranes with further preterm breaking of waters. Since lipid peroxida­ tion (LPO) is ahead of both physiological reactions of synthesis and metabolism of prostaglandins and free radical processes of cellular catabolism, it might be useful to consider LPO and activity of antioxidant system in various versions of the breaking of waters, dependent on the metabolic reactions. The purpose of the research was to study LPO and activity of antioxidant system in fetal membranes in parturient women with preterm and timely breaking of waters. Patients have been divided into 2 groups: 15 women with preterm breaking of waters (PBW, the main group) and 13 parturient women with timely breaking of waters (TBW, the control group). Membranes from both direct spot of their rupture and remote areas have been examined. LPO condition has been judged on the content and accumula­ tion of malonic dialdehyde (MDA) in tissue, and activity of antioxidant system have been judged on catalase blood index and superoxide dismutase (SOD). The analysis of obtained data showed significant elevation of MDA content in membranes in PBW as compared to indices from the control group (р < 0,05). LPO activation was especially evident while estimating the rate of MDA accumulation, regardless of the area of material sampling. In fact, catalase index and SOD content in fetal mem­ branes were similar both in preterm and timely breaking of waters. This indicates about the lack of adequate re­ sponse of the antioxidant protection system on the elevated LPO intensity in PBW, i. e., indicates about weakening of antioxidant system. The latter can be considered as consequence of the long­term chronic hypoxia of tissues. Meanwhile elevation of MDA concentration in fetal membranes indicates about the manifestation of local synthesis of prostaglandins, weakening the strength of fetal membranes. Thus, studies have shown that syndrome of peroxidation is evident in LPO, resulted in tissue hypoxia, distur­ bance of redox reactions and depression of bioantioxidant system, which, probably, promotes premature rupture of fetal membranes and breaking of waters.

lipid peroxidation, preterm breaking of waters, antioxidant system

• акушерство: учебник для вузов / г. М. савельева, р. и. Шалина, л. г. сичинава [и др.]. – М., 2009. – 656 c
• бобырев В. н. специфичность систем антиоксидантной защиты органов и тканей – основа дифференцированной фармакотерапии антиоксидантами / В. н. бобырев, В. Ф. почерняева, с. г. стародубцев // Экспериментальная и клиническая фармакология. – 2005. – т. 57, № 1. – с. 78­86.
• лакин г. Ф. биометрия / г. Ф. лакин. – М.: Высш. школа, 1998. – 293 с.
• Мусаев а. т. диагностика гипоксии плода по данным показателей перекисного окисления липидов и антиокислитель­ ной активности / а. т. Мусаев // педиатрия. – 2004. – № 12. – с. 88­96.
• савченко а. а. система мать­плацента­плод. Вопросы функциональной диагностики / а. а. савченко, н. М. титова, л. а. новикова [и др.]. – красноярск : рио красгу, 2006. – 129 с.
• стальная и. д. Метод определения малонового диальдегида / и. д. стальная, т. г. гаришвили // современные методы в биохимии / под ред. ореховича В. н. – 1997. – C. 66­68.
• Carole B. Rudra A prospective study of early­pregnancy plasma malondialdehyde concentration and risk of preeclampsia / Carole B. Rudra, Chunfang Qiu, Robert M. David at аl. // Clinical Biochemistry, 2006. – Vol. 39. – P. 722­726.
• Tohoku J. Nitric Oxide, Lipid Peroxides, and Uric Acid Levels in Pre­Eclampsia and Eclampsia / J. Tohoku // Biochem. – 2004. – Vol. 15. – P. 87­92.
• Young­Ju Kim Oxidative stress in pregnant women and birth weight reduction / Young­Ju Kim, Yun­Chul Hong, Kwan­Hee Lee // Reproductive Toxicologi, 2005. – Vol. 19. – P. 487­492.

«Bulletin of problems biology and medicine» Issue 3 part 2 (111), 2014 year, 123-125 pages, index UDK 618. 345­008. 811. 1 – 07