Martynenko R. V., Shepitko V. I., Stetsuk Ye. V., Boruta N. V., Rud M. V., Vilkhova O. V.

QUANTITATIVE AND QUALITATIVE CHANGES IN MONOCYTE SPROUT AND RED BONE MARROW MICROENVIRONMENT CELLS UNDER LONG-TERM ADMINISTRATION OF TRYPTORELIN WITH QUERCETIN IN THE EXPERIMENT

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About the author:

Martynenko R. V., Shepitko V. I., Stetsuk Ye. V., Boruta N. V., Rud M. V., Vilkhova O. V.

Heading:

MORPHOLOGY

Type of article:

Scientific article

Annotation:

The study of red bone marrow features and morphological and functional characteristics is relevant because it plays the role of a central organ of haemopoiesis and immunopoiesis. In our study, we focused on the course of monocytopoiesis, since the monocyte sprout is a source of monocytes that proliferate and transform into macrophages in the tissues of the body. In turn, the cells of the environment of RBM consist of many cell types, and the relative impact of each of these cells on the maintenance of a haematopoietic clone remains largely unclear under the influence of various endogenous and exogenous factors on these cells. In contemporary urological practice and according to European Association of Urology's suggestions, Tryptorelin is extensively employed for androgen deprivation therapy. Quercetin, a flavonoid present in fruits and vegetables possesses distinctive biological properties. The administration of tryptorelin with a long-term effect (1 year) and oral administration of the riboflavonoid "Quercetin" leads to statistically significant changes in the quantitative and qualitative composition of RBM, in particular, the studied cells of monocyte sprout and cells of environment. The maximum quantitative changes in MS cells were observed at 3 and 6 months of the study followed by a gradual recovery to the control group. Cells of environment had a significant variability of changes: the quantitative characteristics of macrophages and reticular cells remained constant, but the NSC index for macrophages was lower at all observation periods compared to the control group due to a decrease in nucleus area. Adipocytes responded with a positive increase in quantitative and qualitative characteristics.

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Bibliography:

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Publication of the article:

«Bulletin of problems biology and medicine», 2023 Issue 3, 170, 407-411 pages, index UDC 611.018.52:612.08:615.2

DOI:

10.29254/2077-4214-2023-3-170-407-411