Karlova O. O.

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

Introduction. The definition of the content of diene conjugates (E220), superoxide dismutase (SOD), TBA active oxygen products (TBA-APC) is the one of the numerous diagnostic methods that used to determine the state of the process of free-radical oxidation (FRO) in laboratory practice. Activating the processes FRO is contrib- utes to disturbances in the system of nitric oxide which is determined by the amount of nitrate-nitrite content and nitric oxide Depot – S-nitrosothiols and 2-4 dinitrosyl iron complexes (2-4 DNCI). However, there are significant is- sues related to the low intensity of these indicators in assessing the performance of oxidative stress, nitric oxide of the system and its major metabolites. Objective. To study the predictive value of indicators of oxidative stress and nitric oxide system during lead exposure. Materials and methods. The object of the study were 203 persons, male, aged 38-47 years. 146 electricians of cable networkswas included to the main group, and 57 engineers and technicians – to the control group. Patients were divided into 3 groups depending on the content of lead in the blood. By the 1st group included individuals with dangerous levels of lead levels – 2,12 ± 0,013 mcmol/l; to the second group includes workers with acceptable levels of lead and pronounced asthenic – vegetative symptoms – 1,92 ± 0,013 mcmol/l; to the 3rd group includes employee with acceptable levels of lead and certain symptoms of asthenic-vegetative syndrome – 1,72 ± 0,028 mcmol/l. Results. The studies have shown that increasing of the Pb-K in workers exposed by lead is accompanied by an imbalance in the system of FRO, which manifests by its excessive production in the form of excess of diene con- jugates (E220) and TBK-APk, and decrease in antioxidant protection in the form of reducing of the SOD amount. These changes promote the formation of imbalance in nitric oxide synthesis in the form of increasing of the content the sum of nitrite-nitrate accumulation of products it’s metabolism in the “depot”. It confirmed by increased the levels of indicators the S-nitrosothiols and 2-4 dinitrosyl iron complexes. The threshold levels the studied indexes, which have high specificity and sensitivity, was established. Conclusions. The established the changes of processes lipid peroxidation in workers exposed by lead, which are characterized by changes in processes activation in the system of free radical oxidation on a background of an- tioxidant protection. The mentioned changes accompanied by the formation of an imbalance in the system of nitric oxide with excessive deposition of products of its metabolism. Established that the model the evaluation of relative risk of changes in the parameters of the lipid peroxidation, antioxidant systems and nitric oxide systems are heterogeneous – I2 = 33. 5 %, p = 0.0007.

lead, specificity, sensitivity, threshold levels, nitric oxide free radical oxidation

«Bulletin of problems biology and medicine» Issue 4 part 1 (113), 2014 year, 129-134 pages, index UDK 615. 916’1:546. 815/. 819:616-002