Kuzovkova S. D., Liskina I. V., Zagaba L. M.

MORPHOLOGY

Scentific article

The main condition for the development of a chronic infection, such as pulmonary tuberculoma, is a long persistence of the pathogen in the human body, which can be associated with both the peculiarities of the mycobacterium tuberculosis itself and with impaired local immunity of the macro-organism. Well known that macrophages play the central role both in the host protective immune response and control of infection, and in the maintenance of chronic infection and its associated tissue damage and pathology. Macrophages take part in an early nonspecific inflammatory response to infection, in the formation of a specific immune response, participate in a cellular-mediated immune response, which determines their key role in the eradication of the infection or, conversely, in its development. Immunohistochemical method of detecting mycobacterial antigens allows detects their presence in various cells, including macrophages, in tissue as well as extracellularly. Dynamics and biological significance of mycobacterial antigens in various structures of pulmonary tissue in postprimary tuberculosis of tuberculomas not been well studied. The aim of the study was the analysis and comparison of the expression pattern of mycobacterial antigens and CD68 + cells in various structures of lung tissue with tuberculoma at different activity of tuberculosis inflammation. The material of the study was 34 surgically resected lung segments or lobes with tuberculoma: 19 cases with the high degree and 15 cases with morphologically low degree of activity of specific inflammation. Immunohistochemical study was performed using AUTOSTAINER 360-2D system manufactured by Thermo Fisher Scientific (USA) and reagents its company with UltraVision Quanto imaging system. A polyclonal antibody to M. tuberculosis (Mycobacterium tuberculosis Antibody PA1-7231) manufactured by Pierse (USA) and monoclonal antibody CD68 (Macrophage marker) were used. Microscopic examination of the samples was performed using microscope Olympus BX51, magnifications x100, х400. CD68+ cells and cells with MBT antigens were revealed mainly in capsule of tuberculoma, neighboring areas of distelectasis, and alveoli. It was established that infected macrophages predominate in the capsule of tuberculoma, in the adjacent regions of the distelectase and in the alveoli at a morphologically high degree of activity of the tuberculous inflammatory process. The lowering of the activity of a specific inflammatory process is characterized by both a decrease in the number of cells with the expression of mycobacterial antigens and the number of CD68+ cells in the capsule tuberculoma, alveolar spaces and sites of the distelectase near the capsule.

pulmonary tuberculoma, morphological activity, inflammation, immunohistochemistry

1. Vinokurov II, Argunov AA, Nikolaev YuYa, Plotnikova NV. Kliniko-morfologicheskie osobennosti tuberkulem legkih v usloviyah krajnego severa. Probl. tuberkuleza i boleznej legkih. 2006 Avg;7:44-7. [in Russiаn].
2. Holodok OA, Grigorenko AA, Cheremkin MI. Tuberkulema legkogo kak forma tuberkuleznogo processa. Byul. fiziol. i patol. dyhaniya. 2014 Iyun’;53:126-31. [in Russiаn].
3. Liskina IV, Mel’nik OA. Morfologicheskie osobennosti legochnoj tkani s tuberkulemoj v faze progressirovaniya specificheskogo vospaleniya. East European j. 2016 Fevr;2(6),2:153-60. [in Russiаn].
4. Dorozhkova IR. Vozbuditel’ tuberkuleza: istoriya otkrytiya i izucheniya. Tuberkulez i bolezni legkih. 2012 Maj 03;3:3-14. [in Russiаn].
5. Guirado E, Schlesinger LS, Kaplan G. Macrophages in Tuberculosis: Friend or Foe [Internet]. Semin Immunopathol. 2013 September;35(5):563-83. DOI: 10.1007/s00281-013-0388-2
6. Dedieu L, Serveau-Avesque C, Kremer L, Canaan S. Mycobacterial lipolytic enzymes: a gold mine for tuberculosis research. Biochim. 2013 Jan;1:66-73.
7. Hunter RL. Pathology of post primary tuberculosis of the lung: an illustrated critical review. Tuberculosis (Edinb.). 2011 Nov;91(6):497-509.
8. Karimi S, Shamaei M, Pourabdollah M, Sadr M, Karbasi M, Kiani A, et al. Histopathological findings in immunohistological staining of the granulomatous tissue reaction associated with tuberculosis [Internet]. Tuberculosis Research and Treatment. 2014 Jan. Available from: http:// dx.doi.org/10.1155/2014/858396
9. Ihama Y, Hokama A, Hibiya K, Kishimoto K. Diagnosis of intestinal tuberculosis using a monoclonal antibody to Mycobacterium tuberculosis. World J. Gastroenterol. 2012 Dec 21;18(47):6974-80.
10. Kuzovkova SD, Liskina IV, Mel`ny`k OO, Zagaba LM. Vy`znachennya lokalizaciyi ta xarakteru ekspresiyi mikobakterial`ny`x anty`geniv vidnosno rizny`x struktur legenevoyi tkany`ny` pry` tuberkul`omax legen`. Visn. problem biologiyi i medy`cy`ny`. 2017 Apr 14;2(136):314-8. [in Ukrainian].
11. Liskina IV, Zagaba LM, Oleksinskaya OA. Kliniko-morfologicheskie osobennosti tuberkulem legkih s morfologicheskimi priznakami aktivnosti specificheskogo processa. Perspektivi medicini ta bіologії. 2013 Grud 5;V(2):93-100. [in Russiаn].
12. Lapach SN, Chubenko AV, Babich PN. Statisticheskie metody v mediko-biologicheskih issledovaniyah s ispol’zovaniem Excel. Kiуv: Morion; 2001. 408 s. [in Russiаn].
13. Liskina IV, Mel`ny`k OO. Rozpodil makrofagiv u legenevij tkany`ni pry` tuberkul`omax legen` z riznoyu akty`vnistyu specy`fichnogo zapalennya za rezul`tatamy` imunogistoximichnogo doslidzhennya. Visn. probl. biologiyi i medy`cy`ny`. 2015 Sich 5;1(117):242-8. [in Ukrainian].

«Bulletin of problems biology and medicine» Issue 2 (144), 2018 year, 319-324 pages, index UDK 616-002.592-097:612.215.3:576.852.211

10.29254/2077-4214-2018-2-144-319-324