Vlasenko N. A., Vazhnichaya E. M.

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

Caffeine is one of the most famous substances in the world. The drug is studied in healthy individuals and patients of different ages and gender, under stress and physical exercises, in a wide range of doses and at different times after the administration. There is no clear answer in the literature how it affects the ratio of sympathetic and parasympathetic regulation in the body. Therefore, it is of interest to clarify the effect of caffeine on the autonomic regulation of blood circulation in young healthy volunteers. Research purpose was to study the effect of a single average therapeutic dose of caffeine-sodium benzoate on the Kerdo vegetative index and to assess changes in the tone of the autonomic nerve system. Object and research methods. The study involved 36 volunteer students aged 20-22 years, among them, 20 men and 16 females. The composition of the groups was formed stochastically. All participants were healthy, had no contraindications for caffeine intake and gave their informed consent. They took 1 tablet (200 mg) of caffeinesodium benzoate. Before taking the drug and 40 minutes after it, the respiratory rate, heart rate (HR) and blood pressure (BP) were determined by conventional methods. The Kerdo vegetative index (VI) was calculated. The obtained data were processed using standard computer programs Statistica for Windows 8.0. In relation to the frequency of positive or negative values of VI, the nonparametric criterion “Fisher’s exact method” (TMF) was used. Research results and discussion. Before taking caffeine- sodium benzoate, the respiration rate, HR and BP in all study participants were within normal limits and did not differ in men and women. The average values of VI in both men and women were negative and did not differ significantly, that indicated the predominance of parasympathetic regulation. After taking the drug, an increase in systolic and diastolic BP was observed in males (p <0.05). In this case, HR and respiratory rate did not change compared to the initial level. VI remained negative, but increased in absolute value (p <0.05), which can be regarded as an increase in parasympatheticotonia and its role in the regulation of blood circulation. After taking caffeine-sodium benzoate in women, only an increase in systolic BP was noted (p <0.05). Under the influence of caffeine, gender differences appeared in the respiration rate and VI, which after administration of the drug were lower (p <0.05) in females. A more intense increase in parasympatheticotonia in men was also confirmed by a greater frequency of an increase in the absolute values of negative VIs and (or) their appearance at the place of positive and zero indices (p TMF <0.025) compared to that in women. Thus, with the random formation of groups for a clinical study, it is possible that people prevail in them with the prevalence of one or another part of the autonomic nerve system (in our case, parasympatheticotonia). The increase in the parasympathetic effect in the blood circulation system under the action of caffeine in male volunteers, obviously, reflects the more pronounced activating effect of this substance on the vagus nerve centre on the basis of initial parasympatheticotonia. Conclusion. The results obtained suggest that in studies of the effects of caffeine and its salts on humans, groups should be randomized not only by gender, but also by the state of autonomic regulation, for example, based on the Kerdo VI.

caffeine, heart rate, blood pressure, vegetative index, parasympatheticotonia

1. Mitchell DC, Knight CA, Hockenberry J, Teplansky R, Hartman TJ. Beverage caffeine intakes in the U.S. Food and Chemical Toxicology. 2014 Jan;63:136-42.
2. Temple JL, Bernard C, Lipshultz SE, Czachor JD, Westphal JA, Mestre MA. The Safety of Ingested Caffeine: A Comprehensive Review. Front Psychiatry. 2017 May 26;8:80.
3. McLellan TM, Caldwell JA, Lieberman HR. A review of caffeine’s effects on cognitive, physical and occupational performance. Neurosci Biobehav Rev. 2016;71:294-312.
4. Arnaud MJ. Pharmacokinetics and metabolism of natural methylxanthines in animal and man. Handb Exp Pharmacol. 2011;200:33-91.
5. Skinner TL, Jenkins DG, Leveritt MD, McGorm A, Bolam KA, Coombes JS, et al. Factors influencing serum caffeine concentrations following caffeine ingestion. J Sci Med Sport. 2014;17:516-20.
6. Bobyrov VM, Vazhnycha OM, Deviatkina TO, Lutsenko RV, Deviatkina NM. Farmakolohiia: praktykum: navchalnyi posibnyk dlia studentiv vyshchykh medychnykh navchalnykh zakladiv. Vinnytsia: Nova Knyha; 2017. 352 s. [in Ukrainian].
7. Yang A, Palmer AA, de Wit H. Genetics of caffeine consumption and responses to caffeine. Psychopharmacology (Berl). 2010;211:245-57.
8. Riksen NP, Smits P, Rongen GA. The cardiovascular effects of methylxanthines. Handb Exp Pharmacol. 2011;200:413-37.
9. Gonzaga LA, Vanderlei LCM, Gomes RL, Garner DM, Valenti VE. Involvement of Cardiorespiratory Capacity on the Acute Effects of Caffeine on Autonomic Recovery. Medicina (Kaunas). 2019 May 23;55(5):E196.
10. Flueck JL, Schaufelberger F, Lienert M, Schäfer Olstad D, Wilhelm M, Perret C. Acute Effects of Caffeine on Heart Rate Variability, Blood Pressure and Tidal Volume in Paraplegic and Tetraplegic Compared to Able-Bodied Individuals: A Randomized, Blinded Trial. PLoS One. 2016 Oct 24;11(10):e0165034.
11. Crooks E, Hansen DA, Satterfield BC, Layton ME, Van Dongen HPA. Cardiac autonomic activity during sleep deprivation with and without caffeine administration. Physiol Behav. 2019 Aug 6;210:112643.
12. Monda M, Viggiano A, Vicidomini C, Viggiano A, Iannaccone T, Tafuri D, et al. Espresso coffee increases parasympathetic activity in young, healthy people. Nutr Neurosci. 2009 Feb;12(1):43-8.
13. Instruktsiia do medychnoho zastosuvannia likarskoho zasobu kofein-benzoat natriiu. UA/6199/01/01(22908) [Internet]. Likykontrol, 2019. [Onovleno 2019, tsytovano 2019 Ver.18]. Dostupno: likicontrol.com.ua http://likicontrol.com.ua/%D1%96%D0%BD%D1%81%D1%82%D1%8 0%D1%83%D0%BA%D1%86%D1%96%D1%8F/?[22908] [in Ukrainian].
14. Muhin NA, Moiseev BC. Propedevtika vnutrennih bolezney: uchebnik. 2-e izd., dop. i pererab. Moskva: GEOTAR-Media; 2008. 848 s. [in Russiаn].
15. Kérdö I. Ein aus Daten der Blutzirkulation kalkulierter Index zur Beurteilung der vegetativen Tonuslage. Acta neurovegetativa. 1966;29(2):250-68.
16. Gubler EV, Genkin AA. Primenenie neparametricheskih kriteriev statistiki v mediko-biologicheskih issledovaniyah. Izd. 2-e. Leningrad: Meditsina; 1973. 141 s. [in Russiаn].
17. Hibino G, Moritani T, Kawada T, Fushiki T. Caffeine enhances modulation of parasympathetic nerve activity in humans: quantification using power spectral analysis. J Nutr. 1997 Jul;127(7):1422-7.

«Bulletin of problems biology and medicine» Issue 4 Part 1 (153), 2019 year, 75-78 pages, index UDK 616.12-008:615.22

10.29254/2077-4214-2019-4-1-153-75-78