Basysta K. I., Rodinskiy A. G., Demchenko O. М., Guz L. V.

PHYSIOLOGY

Scentific article

The paper presents the results of studying the content of neurotransmitter amino acids in the brain and hippocampus of rats of different ages (middle-aged rats and old rats) with experimental hyperglycemia. The animals were kept in standard vivarium conditions in the amount of 90 individuals. Each age group was divided into two subgroups: intact and experimental with simulated experimental hyperglycemia. Experimental hyperglycemia was modeled by intraperitoneal administration of aloxane monohydrate solution (120 mg / kg, “Sigma”). Blood glucose was determined using a Bionime portable glucometer for 10 days. On day 10, those animals that had a blood glucose level higher than 28 mmol/l were selected. Behavioral and emotional changes in behavioral tests, such as «Open Field», «CRPA», «Raised cruciform labyrinth», were first studied. After the tests, rats were selected that showed the most significant results for determining the content of GABA, glycine, serotonin, glutamate in the cortex of the large hemispheres and the hippocampus. To collect brain tissue, the animals were euthanized, decapitated, tissue isolated, treated with liquid nitrogen, crushed and examined for the content of neurotransmitter amino acids by thin layer chromatography. Behavioral tests in middle-aged and elderly rats with hyperglycemia revealed significant disturbances in the organization of cognitive function during gerontogenesis. In middle-aged rats against this background, there was a significant increase in glutamate in the cortex and hippocampus. In older rats, an increase in glutamate levels in the neocortex and hippocampus was observed against a background of less pronounced behavioral and cognitive deficits. The content of glycine, GABA, serotonin in the neocortex of old rats, as in middle-aged rats remained at the level of control values. Thus, a significant behavioral disorder in both age groups is provided by the development of the exototoxic effect of glutamate in the neocortex and hippocampus. Cognitive deficiency, mainly in middle-aged animals, with excessive accumulation of serotonin in the hippocampus.

experimental hyperglycemia, diabetes mellitus, alloxan-induced diabetes, behavioral activity, memory, serotonin, GABA, glutamate, cerebral cortex, hippocampus, neurotransmitter amino acids.

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«Bulletin of problems biology and medicine» Issue 3 (161), 2021 year, 356-359 pages, index UDK 616.153.455-008.61:616.831]-076-092.9-053

10.29254/2077-4214-2021-3-161-356-359