NATURAL ORIGIN IMMUNOMODULATORS INFLUENCE ON PROTEIN FRACTIONS, CORTISOL AND GLUTATHIONE CONTENT IN BROILER CHICKENS BLOOD UNDER STRESS

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  3. NATURAL ORIGIN IMMUNOMODULATORS INFLUENCE ON PROTEIN FRACTIONS, CORTISOL AND GLUTATHIONE CONTENT IN BROILER CHICKENS BLOOD UNDER STRESS
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Publication of the article:

«Bulletin of problems biology and medicine», 2014 Issue 4 part 1, 113,

Grabovskyi S. S. , Grabovska O. S.

Natural Origin Immunomodulators Influence on Protein Fractions, Cortisol and Glutathione Content in Broiler Chickens Blood under Stress

About the author:

Grabovskyi S. S. , Grabovska O. S.

Heading:

BIOLOGY

Type of article:

Scentific article

Annotation:

The results of determination of protein fractions, cortisol and glutathione content in blood of broiler chickens, which further added to the feed of natural origin biologically active substances are presented in the article. As an antistressors and immunomodulators in pre-slaughter period are using of spleen extract biologically ac- tive substances were obtained with and without ultrasound application. The purpose of research – determination of changes of protein fractions, cortisol and glutathione content in broiler chickens blood before slaughter and their correction of natural origin biologically active substances (spleen extract). Object and research methods. The experiment was conducted on 15 broiler chickens with standard diet. Three groups of broiler chickens one month of age (5 chickens each) was formed for research. The spleen extract was obtained with (I research group) and without ultrasound application (II research group), were using as an biologically active substances to the feed broiler chickens in pre-slaughter period (five days before slaughter). The extracts were applied to feed by aerosol method (70 ° alcohol solution of spleen extract volume of 1.4 ml per chicken). The control group chickens (group III) received to the feed in the same way of 70 ° alcohol solution in the same volume. The feed eating by broiler chickens was exercised daily. The broiler chickens ate food completely. The broiler chickens slaughter was carried out in the morning. The blood plasma protein fractions separation was carried out by horizontal electrophoresis in polyacrylamide gel (PAAG). Cortisol level were determined in broiler chickens blood plasma. Glutathione concentration were determined in broiler chickens blood. Mathematical treatment of the research results worked statistically using the software package Statistica 6.0 and Microsoft Excel for Windows XP. Probability differences was assessed by Student t-test and results considered likely at P 0.05. Results and discussion. We measured the ratio of blood plasma protein fractions of broiler chickens, which in addition to the feed fed of natural origin biologically active substances. As a result of research was found that aerosol introduction of the spleen extract to the broiler chickens feed increases the γ-globulin concentration in broiler chickens blood plasma of the two experimental groups compared to the control. Cortisol level in broiler chickens (which further added to the feed of spleen extract obtained with ultrasound ap- plication (I research group) blood plasma was reliable lower (Р < 0. 05) compared to control, that may to indicate decreasing of stress before slaughter. The free cortisol level decreasing in broiler chickens blood plasma, perhaps, is associated with increasing of globulin fraction content. The glutathione level was found reliable higher (Р < 0. 05) in the broiler chickens blood of the control group. On the poultry feeding final stage is necessary to consider a pre-slaughter stress and to apply of natural origin biologically active substances. The results which obtained can to use in researches of cell immunity indices and stress hormones, such as cortisol, on farm animals for organism resistance increasing, correction and avoid their pre-slaughter stress and improve product quality.

Tags:

broiler chickens, pre-slaughter stress, spleen extract, ultrasound, protein fractions, cortisol, glutathione

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Publication of the article:

«Bulletin of problems biology and medicine» Issue 4 part 1 (113), 2014 year, 57-61 pages, index UDK 636. 52/. 58:519. 11:612. 176

2014 Issue 4 part 1, 113
29.03.2023
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