Hnatyuk M. S., Chernetsky A. A., Tatarchuk L. V., Stets N. Ya.
MORPHOMETRIC ASPECTS OF THE STUDY OF MORPHOLOGICAL CHANGES IN VENOUS VESSELS OF THE HEMOMICROCIRCULATORY BED OF THE THYROID GLAND OF WHITE RATS WITH EXPERIMENTAL POSTOPERATIVE PORTAL HYPERTENSION
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About the author:
Hnatyuk M. S., Chernetsky A. A., Tatarchuk L. V., Stets N. Ya.
Heading:
MORPHOLOGY
Type of article:
Scientific article
Annotation:
The thyroid gland is an important endocrine organ whose hormones regulate most of the essential functions of the body. This organ is considered highly vulnerable to the effects of various adverse endogenous and exogenous factors, as well as to changes in blood flow in the systemic circulation, the portal vein, and the pulmonary artery. The postcapillary venules and venules of the thyroid gland under conditions of elevated pressure in the portal system remain insufficiently studied. Therefore, a detailed and comprehensive investigation of the venous microvessels of the thyroid gland under conditions of increased pressure in the portal vein is necessary. The venous microvessels of the thyroid gland were examined using a complex of morphological and morphomet ric methods in 62 white rats, which were divided into three groups. The first group included 15 control animals after laparotomy, the second group consisted of 30 rats with increased pressure in the portal vein, and the third group included 17 animals with postresection portal hypertension, in which multiple organ failure developed. Increased pressure in the portal vein was induced by resection of the left and right lateral lobes of the liver. One month after the beginning of the experiment, the rats were euthanized by bloodletting under anesthesia. In histological specimens and semithin sections of the studied organ, the area of postcapillary venules and ve nules, the area of their lumens and walls, the indices of these microvessels, and the volume of damaged endothelial cells were measured. Statistical analysis of the obtained data was performed. It was established that the removal of the left and right lateral lobes of the liver in rats was complicated by in creased pressure in the portal vein and significant remodeling of the venous microvessels of the studied organ. These changes were characterized by pronounced dilation of postcapillary venules and venules, an increase in the indices of these microvessels, a decrease in the area of their walls, an increase in the volume of damaged endothelial cells and their dysfunction, impaired venous drainage of the organ, hypoxia, apoptosis, dystrophy, necrobiosis of tissues and cells, and the presence of foci of cellular infiltration and sclerosis. The identified morphological changes of the postcapillary venules and venules of the studied organ were most pronounced under the combined effect of post resection portal hypertension and multiple organ failure in rats.
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Bibliography:
- Ferrari SM, Fallahi P, Antonelli A, Benvenga S. Environmental problems in thyroid diseases. Front Endocrinol. 2017;8:50-54.
- Volchenko IV, Lykhman VM, Skoryy DI, Shevchenko AM. Osoblyvosti vykonannia obshchyrnykh rezektsii pechinky z urakhuvanniam profilaktyky pisliaoperatsiinykh uskladnen. Kharkivska khirurhichna shkola. 2016;3(78):35-39. [in Ukrainian].
- Zatsarynnyi RA, Pidopryhora OO. Kontsentratsiia lidokainu v krovi pry vnutrishnovennomu ta epiduralnomu Vvedenni pry obshyrnykh rezektsiiakh pechinky. Shpytalna khirurhiia. Zhurnal imeni L. Ya. Kovalchuka. 2022;3:20-26. [in Ukrainian].
- Lebrec D, Vinel J, Dupas J. Complications of Portal Hypertension in Adults: A French Consensus. European Journal of Gastroenterology and Hepatology. 2005;17:403-410. DOI: 10.1097/00042737-200504000-00003.
- McConnell M, Iwakiri Y. Biology of portal hypertension. Hepatol. Int. 2018;12:11-23.
- Hnatjuk MS, Protsailo OM, Tatarchuk LV, Monastyrska NJa. Mistsevi imunni reaktsiyi u tovstiy kyshtsi v umovakh postrezektsiynoyi portal noyi hipertenziyi. Klinichna anatomiya ta operatyvna khirurhiya. 2021;20(2):42-47. [in Ukrainian].
- Suvarna SK, Layton C, Bancroft GD, editors. Bancroft’s Theory and Practice of Histological Techniques. 8th ed. Amsterdam: Elsevier; 2019. 558 p. DOI: 10.1016/B978-0-7020-6864-5.00008-6.
- Hayat МА. Principles and techniques of electron microscopy: Biological applications. 4th ed. Cambridge: Cambridge University Press; 2000. 543 p. DOI: 10.1006/anbo. 2001.1367.
- Petrie A, Sabin C. Medical statisties at a Glance. 4th ed. New York: Wiley; 2019. 208 p.
- Council of Europe. European convention for protection of vertebrate animals used for experimental and other scientific purposes. Strasbourg: Council of Europe;1986. 52 p.
- European Union. Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the Protection of Animals Used for Scientific Purposes. Off J Eur Union. 2010;53(L276):33-79.
Publication of the article:
«Bulletin of problems biology and medicine», 2026 Issue 1, 180, 425-432 pages, index UDC 616.36-089.87-06.616-91]-092.9
