Pshychenko V. V., Cherno V. S., Chebotar L. D., Yulevich O. I., Shevchuk N. P.

MORPHOLOGICAL CHANGES OF THE PINEAL GLAND OF RATS UNDER CHRONIC INTOXICATION WITH LEAD AND CORRECTION WITH SODIUM SELENITE

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About the author:

Pshychenko V. V., Cherno V. S., Chebotar L. D., Yulevich O. I., Shevchuk N. P.

Heading:

MORPHOLOGY

Type of article:

Scientific article

Annotation:

The rapid development of industry has led to a progressive increase in the level of environmental pollution and the impact of a significant amount of heavy metals on the body. Lead and its salts are one of the most common and toxic heavy metals present in the environment. Lead intoxication is accompanied by signs of desynchronization and disruption of adaptation processes. Lead intoxication is accompanied by signs of desynchronization and disruption of adaptation processes. Since the pineal gland is actively involved in adaptation processes and ensures the maintenance of homeostasis, the purpose of the study was to study the morphological changes of the pineal gland under the conditions of intoxication with lead acetate and correction with sodium selenite. The research was conducted on 18 male Wistar rats weighing 200-240 g. Animals of the 1st (control) group were kept under normal vivarium conditions without the influence of additional factors. Animals of the II group were simulated lead acetate intoxication daily for 30 days by intragastric administration at a dose of 0.3 mg/kg. For 30 days, animals of the III group were simulated lead acetate intoxication and intraperitoneally administered sodium selenite (Na2 SeO3 ) at the rate of 0.2 mg/kg. As the analysis of the obtained results showed, there is a pronounced, toxic effect of lead acetate on the morphology of the pineal gland, which is accompanied by the restructuring of the peripheral and subcapsular region of the parenchyma of the organ, due to the development of hydropic dystrophy and necrosis of light cells. It was found that sodium selenite reduces the toxic effect of lead acetate on pinealocytes, promotes the restoration of synthetic and secretory activity of cells, reduce signs of edema and dystrophic changes. The protective effect of sodium selenite is also indicated by the increase in the total number of pinealocytes and the approximation of their quantitative content to the control indicators, which indicates the restoration of the cellular composition of the pineal parenchyma and the reduction of the manifestations of necrosis. Sodium selenite is a potentially useful agent for the pharmacological correction of pathological changes in the pineal gland caused by chronic exposure to lead acetate.

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Bibliography:

1. Yuan W, Yang N, Li X. Advances in Understanding How Heavy Metal Pollution Triggers Gastric Cancer. BioMed Research International. 2016;1:1-10.
2. Stepanchuk VV. Ontohenetychni osoblyvosti tsyrkadiannykh khronorytmiv vilʹnoradykalʹnoho homeostazu za umov svyntsevoho otruyennya. Klinichna ta eksperymentalʹna patolohiya. 2014;13(2):131-133. [in Ukrainian].
3. Chebotar LD, Tsvyakh OO, Larycheva OM. Orhanni efekty melatoninu. Mykolayiv: Politekhnika; 2018. 235 s. [in Ukrainian].
4. Shen W, Chen J, Yin J, Wang S-L. Selenium protects reproductive system and foetus development in a rat model of gestational lead exposure. Eur Rev Med Pharmacol Sci. 2016;20(4):773-80.
5. Melʹnykova NM, Voroshylova NM. Vplyv selenitu natriyu ta vitaminu E na vmist deyakykh mineralʹnykh elementiv u krovi shchuriv otruyenykh kadmiyem. V: Anna Svoboda, redaktor. Proceedings of the 8th International Scientific and Practical Conference Scientific Horizon in The Context of Social Crises; 2021 April 11-12; Tokyo. Japan: Otsuki Press; 2021. p. 375-380. [in Ukrainian].
6. Joshee L, Kiefer A, Seney C, Matta KE, Orr SE, Bridges CC. Prophylactic supplementation with selenium alters disposition of mercury in aged rats. Exp Gerontol. 2021 Jul 1;149:111-289.
7. Milošević MD, Paunović MG, Matić MM, Ognjanović BI, Saičić ZS. Role of selenium and vitamin C in mitigating oxidative stress induced by fenitrothion in rat liver. Biomed Pharmacother. 2018 Oct;106:232-238.
8. Ansar S. Effect of Selenium on the Levels of Cytokines and Trace Elements in Toxin-Mediated Oxidative Stress in Male Rats. Biol Trace Elem Res. 2016 Jan;169(1):129-33.
9. Slobodyan SO, Hutyy BV, Mursʹka SD. Vplyv selenitu natriyu i kormovoyi dobavky «Metisevit plyus» na morfolohichni pokaznyky krovi shchuriv za intoksykatsiyi Kadmiyem i Svyntsem. Naukovyy visnyk LNUVMB imeni S.Z. Gzhytsʹkoho. Seriya: Veterynarni nauky. 2020;22(97): 52-57. [in Ukrainian].
10. Hordiyenko VV, Kosuba RB, Perepelytsya OO. Fitokhimichna korektsiya nakopychennya vazhkykh metaliv za umov eksperymentalʹnoho metalotoksykozu. Klinichna ta eksperymentalʹna patolohiya. 2017;1(59):68-71. [in Ukrainian].
11. Ma W, He S, Ma H, Jiang H, Yan N, Zhu L, et al. Nanoparticle Exposure Causes Pulmonary Structural Damage and Mitochondrial Dynamic Imbalance in the Rat: Protective Effects of Sodium Selenite. Int J Nanomedicine. 2020 Jan 30;15:633-645.

Publication of the article:

«Bulletin of problems biology and medicine», 2023 Issue 1, 168, 325-330 pages, index UDC 591.481.3

DOI:

10.29254/2077-4214-2023-1-168-325-330