Shevchuk M. М., Volos L. I.

MORPHOLOGICAL AND MORPHOMETRIC CHANGES IN THE HEART AND CORONARY VESSELS OF RATS AFTER 2 WEEKS OF EXPERIMENTAL APPLICATION OF 10% CANNABIDIOL OIL

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About the author:

Shevchuk M. М., Volos L. I.

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MORPHOLOGY

Type of article:

Scientific article

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Cannabidiol (CBD) is a natural non-psychoactive extract of Cannabis sativa L, with therapeutic effects, in the treatment of cardiovascular diseases. The aim of the study was to determine morphological and morphometric changes in the heart and coronary vessels of rats after 2 weeks of experimental application of 10% CBD oil. The ex periment was conducted on 26 sexually mature white male rats weighing 180-230 g in compliance with bioethical standards. The animals of the experimental group (14) received 10% CBD oil orally once a day at a dose of 10 mg/ kg for 2 weeks with the standard diet. The animals of the control group (6) received the CBD carrier solvent - hemp seed oil orally once a day at a dose of 0.1 ml/kg for 2 weeks with the standard diet. The intact group consisted of 6 rats that were only on the standard diet. Morphological features of the heart were determined, morphometry of heart vessels were performed, their diameters and wall thickness with statistical data processing. It was found that the morphological structure of the myocardium and coronary vessels retained typical histoarchitectonics without signs of destructive, inflammatory or sclerotic changes. Morphometric parameters of large-caliber coronary arteries did not significantly differ from the control group (p>0.05). In small arteries, a predominance of dilatational changes without signs of remodeling was observed. Morphometric parameters of arterioles in the CBD group showed the profile of functional vasodilation without structural reorganization of the vascular wall. Myocardial capillaries were characterized by preserved structural organization, intact endothelial layer and stable density of the capillary net work. No signs of reduction of the microcirculatory bed or impaired perfusion were detected.

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Bibliography:

1. Atalay S, Jarocka-Karpowicz I, Skrzydlewska E. Antioxidative and Anti-Inflammatory Properties of Cannabidiol. Antioxidants (Basel). 2019;9(1):21. DOI: 10.3390/antiox9010021.
2. Urlić H, Kumrić M, Pavlović N, Dujić G, Dujić Ž, Božić J. Cardiovascular Effects of Cannabidiol: From Molecular Mechanisms to Clinical Implementation. Int J Mol Sci. 2025;26(19):9610. DOI: 10.3390/ijms 26199610.
3. Guo Y, Wei R, Deng J, Guo W. Research progress in the management of vascular disease with cannabidiol: a review. J Cardiothorac Surg. 2024;19(6):1-11. DOI: 10.1186/s13019-023-02476-y.
4. Nguyen C, Moeller KE, McGuire M, Melton BL. Consumer perception, knowledge, and uses of cannabidiol. Ment Health Clin. 2023;13(5):217 224. DOI: 10.9740/mhc.2023.10.217.
5. García-Rivas G, Lozano O, Bernal-Ramírez J, Silva-Platas C, Salazar-Ramírez F, Méndez-Fernández A, et al. Cannabidiol Prevents Heart Failure Dysfunction and Remodeling Through Preservation of Mitochondrial Function and Calcium Handling. JACC Basic Transl Sci. 2025;10(6):800-821. DOI: 10.1016/j.jacbts.2024.12.009.
6. Baranowska-Kuczko M, Kozłowska H, Kloza M, Sadowska O, Kozłowski M, Kusaczuk M, et al. Vasodilatory effects of cannabidiol in human pulmonary and rat small mesenteric arteries: modification by hypertension and the potential pharmacological opportunities. J Hy pertens. 2020;38(5):896-911. DOI: 10.1097/HJH.0000000000002333.
7. Kicman A, Toczek M. The Effects of Cannabidiol, a Non-Intoxicating Compound of Cannabis, on the Cardiovascular System in Health and Disease. Int J Mol Sci. 2020;21(18):6740. DOI: 10.3390/ijms21186740.
8. Sultan SR, Millar SA, England TJ, O’Sullivan SE. A Systematic Review and Meta-Analysis of the Haemodynamic Effects of Cannabidiol. Front Pharmacol. 2017;8:81. DOI: 10.3389/fphar.2017.00081.
9. Jadoon KA, Tan GD, O’Sullivan SE. A single dose of cannabidiol reduces blood pressure in healthy volunteers in a randomized crossover study. JCI Insight. 2017;2(12):e93760. DOI: 10.1172/jci.insight.93760.
10. Council of Europe. European Convention for the protection of vertebrate animals used for experimental and other scientific purposes. Strasburg: Council of Europe; 1986; 52 p.
11. European Union. Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the Protection of Animals Used for Scientific Purposes. Off J Eur Union. 2010;53(L276):33-79.
12. Suvarna SK, Layton C, Bancroft GD, editors. Bancroft’s Theory and Practice of Histological Techniques. 8th ed. Amsterdam: Elsevier; 2019. 558 p. DOI: 10.1016/B978-0-7020-6864-5.00008-6.
13. Hayat МА. Principles and techniques of electron microscopy: Biological applications. 4th ed. Cambridge: Cambridge University Press; 2000. 543 p. DOI: 10.1006/anbo.2001.1367.
14. Magaki S, Hojat SA, Wei B, So A, Yong WH. An Introduction to the Performance of Immunohistochemistry. Methods Mol Biol. 2019;1897:289 98. DOI: 10.1007/978-1-4939-8935-5_25.
15. Nguyen T. Immunohistochemistry: A Technical Guide to Current Practices. Cambridge: Cambridge University Press; 2022. 272 p.
16. Hruzieva TS, Lekhan VM, Ohniev VA, Haliienko LI, Kriachkova LV, Palamar BI, ta in. Biostatystyka. Vinnytsia: Nova knyha; 2020. 384 s. [in Ukrainian].
17. Hooshmand SAA, Rameshrad M, Sahebkar A, Iranshahi M. The effects of cannabidiol on nitric oxide synthases: a narrative review on therapeutic implications for inflammation and oxidative stress in health and disease. J Cannabis Res. 2025;7(1):71. DOI: 10.1186/s42238 025-00332-5.
18. Stanley CP, Hind WH, Tufarelli C, O’Sullivan SE. Cannabidiol causes endothelium-dependent vasorelaxation of human mesenteric arteries via CB1 activation. Cardiovasc Res. 2015;107(4):568-78. DOI: 10.1093/cvr/cvv179.
19. O’Sullivan SE. An update on PPAR activation by cannabinoids. Br J Pharmacol. 2016;173(12):1899-910. DOI: 10.1111/bph.13497.

Publication of the article:

«Bulletin of problems biology and medicine», 2026 Issue 1, 180, 478-488 pages, index UDC 616.12+616.132.2)]-018: [615.34: 582.635.38] -019-092.9

DOI:

10.29254/2077-4214-2026-1-180-478-488