Myrnyi V. H., Moisieieva N. M.
MODULATION OF NITROGEN METABOLISM UNDER CHRONIC COLD STRESS IN GUINEA PIGS: THE ROLE OF DALARGIN AND NALOXONE IN MEDIATING A PROTECTIVE EFFECT
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About the author:
Myrnyi V. H., Moisieieva N. M.
Heading:
BIOLOGY
Type of article:
Scientific article
Annotation:
Adaptation to cold stress is a critical process for maintaining viability under extreme low-temperature conditions. This process activates complex neurohumoral mechanisms that ensure thermogenesis, homeostasis, and functional stability of the organism. One of the key research areas is the study of synthetic neuropeptides, such as dalargin, which are capable of reducing cellular stress, preventing apoptosis, and promoting cell proliferation. However, the effects of this peptide on key markers of nitrogen metabolism, such as creatinine and urea, under prolonged cooling conditions remain insufficiently studied. The aim of this work was to assess the impact of dalargin on nitrogen metabolism markers (urea and creatinine) in guinea pigs under chronic cold stress (CCS) and to investigate the mechanisms underlying its protective effects. The experiment was conducted on guinea pigs, which were subjected to CCS through reduced ambient temperature. The results showed that CCS caused an increase in urea levels and a decrease in creatinine levels, indicating disturbances in nitrogen metabolism, enhanced catabolism, and impaired kidney function. Administration of dalargin led to a reduction in urea levels, but creatinine levels remained similar to those of the CCS group, indicating the selective nephroprotective effect of dalargin. To further explore the mechanisms of this action, the synthetic neuropeptide antagonist, naloxone, was used. The results demonstrated that naloxone administration partially reduced urea levels but did not restore the parameters to control levels. The data obtained confirm the role of opioid regulation in cold stress adaptation and indicate the potential of dalargin to cor- rect nitrogen metabolism disturbances under CCS. These findings open prospects for the use of neuropeptides in the treatment of metabolic disorders under extreme temperature conditions.
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Publication of the article:
«Bulletin of problems biology and medicine», 2025 Issue 3,178, 122-128 pages, index UDC 577.12.084:612.592:615.21
