Nakonechna O. A., Posokhov Y. O., Bezrodnaya A. I., Yarmysh N. V., Bachynskyi R. O.

INVESTIGATION OF THE CONDITION OF THE ERYTHROCYTE MEMBRANES OF RATS USING A FLUORESCENT PROBE UNDER CONDITIONS OF EXPOSURE TO YTTRIUM GADOLINIUM ORTHOVANADATE NANOPARTICLES AND POLYETHYLENE GLYCOL-400

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Nakonechna O. A., Posokhov Y. O., Bezrodnaya A. I., Yarmysh N. V., Bachynskyi R. O.

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NANOMEDICINE AND NANOTECHNOLOGY

Type of article:

Scientific article

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Nanomaterials based on rare earth metals have become widely used in medicine for radiotherapy for patients with cancer. However, irradiation of nanoparticles (NP) generates many short-range electrons, which can cause their modification. Polyethylene glycol-400 (PEG-400) is a cell-impermeable substance that can adsorb on the membrane surface, causing changes in its structure and charge value, being incorporated into its hydrophobic regions and interacting with the fatty acid tails of phospholipids or hydrophobic regions of integral proteins. The fluorescent probe O1O (2-(2-hydroxy-phenyl)-5-phenyl-1,3-oxazole) was used to study the state of rat erythrocyte membranes under the influence of NP (with and without irradiation) and PEG-400, whose fluorescence parameters depend on the polarity and proton donor capacity of its microenvironment. Since an increase in the hydration of the lipid bilayer of cell membranes leads to an increase in the proton donor capacity and polarity of the membrane environment, the O1O probe can be used to detect changes in the hydration of lipid membranes. An experimental study of the fluorescence spectrum of the O1O probe in a suspension of rat erythrocytes exposed to polyethylene glycol-400 indicates the formation of an additional shell of its molecules on the surface of the membranes. The study of the fluorescence spectrum of the O1O probe in the suspension of erythrocytes from rats exposed to NP of yttrium gadolinium orthovanadate activated with europium at doses of 100 μg/kg and 200 μg/kg, both under conditions of their preliminary UV irradiation and without it, did not reveal any changes in the erythrocyte membranes.

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Publication of the article:

«Bulletin of problems biology and medicine», 2024 Issue 3, 174, 341-350 pages, index UDC 577.336:57.084:546.662-022.532

DOI:

10.29254/2077-4214-2024-3-174-341-350