Semenenko I. V., Lukianenko D. R., Zubareva I. M., Sklyar T. V.

INTENSIFICATION OF BIOSYNTHETIC ACTIVITY OF STREPTOMYCES SP. 31

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About the author:

Semenenko I. V., Lukianenko D. R., Zubareva I. M., Sklyar T. V.

Heading:

MICROBIOLOGY

Type of article:

Scientific article

Annotation:

This work focuses on the necessity and possibility of increasing the biosynthetic activity of the streptomycete strain Streptomyces sp. 31, which is obtained from ordinary chernozem soils. The need to study this strain is explained by the fact that it is considered as a potential producer of biologically active substances – stimulators of metabolic processes in living organisms. This strain has proven antagonistic properties against phytopathogenic bacteria and fungi. However, an urgent issue is the study of its biosynthetic properties in relation to the complex of lytic enzymes, which are inducers of a positive effect on the cells of plants, animals, edible mushrooms, etc. Therefore, it is proposed to intensify the development of the Streptomyces sp strain 31. The biosynthetic activity of microorganisms depends on the composition of the nutrient medium. Therefore, it is proposed to study the influence of tryptophan, threonine, arginine and glutamic acid as stimulators of biomass growth, activity of staphylolytic enzymes and the intensity of protein synthesis by the studied strain. According to the specified parameters, the control of the results of the experiments was carried out. It was established that threonine at a concentration of 100 μg/ml increased protein yield by 43% compared to the control. Glutamic acid at a concentration of 200 μg/ml had the most significant effect on biomass growth, increasing the amount of biomass by 86% relative to the control. A similar effect was observed in the presence of threonine (on average, by 27.5%) and arginine (by 85%). All four amino acids had a positive effect on the activity of staphylolytic enzymes, arginine at a concentration of 50 μg/ml had the greatest effect, increasing the activity of staphylolysins by 58%.

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Bibliography:

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Publication of the article:

«Bulletin of problems biology and medicine», 2023 Issue 4, 171, 307-315 pages, index UDC 579.22+577.15

DOI:

10.29254/2077-4214-2023-4-171-307-315