Kyznetsova M. O.

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

Hepatic disorders rank among leading diseases in the structure of morbidity and mortality of population in Ukraine. One of the main factors of liver damage is improper nutrition regimen with protein and carbohydrate deficiency. Data of scientific studies of hyponutrition’s influence on morphofunctional state of the liver in rats’ offspring are rather insufficient and often contradictive. The aim of the work was to study morphofunctional state of the liver in rats’ offspring born of females on lowprotein diet with carbohydrate deficiency during their pregnancy. Morphofunctional state of the liver in rats’ offspring born of females on low-protein diet with carbohydrate deficiency during their pregnancy has been studied. In order to determine morphofunctional state of the liver, a complex of morphometrical and immune histochemical examinations of liver tissues, as well as biochemical studies of blood serum and liver homogenate have been carried out. It is determined that low-calorie diet in rats during pregnancy has a significant influence on liver morphology in their offspring in the form of marked discomplexation of radial lobular structure, increased splenoportal index due to increased stromal and decreased parenchymal liver components, as well as decreased expression of endothelial synthase. Dysproteinemia, hyperlipidemia (increased levels of cholesterol, LDL, VLDL, and TG), hypoglycemia and increased calcitonin were determined in rat offspring serum. A biochemical study of liver homogenate determined signs of disturbed secretion and lipid reuptake manifested as increased cholesterol, accumulation of TG and unesterified fatty acids, as well as decreased level of phospholipids, which is a risk factor for development of liver steatosis and liver cirrhosis.

liver, morphology, homogenate, blood serum, low-protein diet, pregnancy

1. Belousov YuV. Epidemiologiya i faktory riska razvitiya zabolevanij pishchevaritel’noj sistemy u detej. Zdorovia Ukrainy. Tematychnyi nomer: dytiacha hastroenterolohiia. 2012;48-51. [in Russiаn].
2. Sazonova E. Bolezni pecheni v praktike klinicista. Provizor. 2007;5:12-5. [in Russiаn].
3. Abaturov AE, Morozov MS. Vliyanie ehkzogennyh faktorov na genomnyj imprinting. Zdorov’e rebenka. 2016;5(73):170-2. [in Russiаn].
4. Fall CH. Fetal programming and the risk of noncommunicable disease. Indian J. Pediatr. 2013;80 Suppl. 1:13-20.
5. Gluckman PD, Hanson MA, Cooper C, Thornburg KL. Effect of in utero and early-life conditions on adult health and disease. N. Engl. J. Med. 2008;359(1):61-73.
6. Slieker RC, Roost MS, van Iperen L. DNA methylation landscapes of human fetal development. PLoS Genet. 2015;11(10):E10055-83. DOI: 10. 1371/journal. pgen. 1005583
7. Lecoutre S, Breton C. Maternal nutritional manipulations program adipose tissue dysfunction in offspring. Front Physiol. 2015;6:158. DOI: 10.3389/fphys.2015.2015.00158
8. Thornburg KL, Shannon J, Thuillier P, Turker MS. In utero life and epigenetic predisposition for disease. Adv. Genet. 2010;71:57-78.
9. Hernandez –Valero MA, Rother J, Gorlov I, Frazier M, Gorlova O. Interplay between polymorphisms and methylation in the H19/IGF2 gene region may contribute to obesity in mexican-american children. J. Dev. Orig. Health Dis. 2013;4(6):499-506.
10. Williams-Wyss O, Zhang S, Mac Laughlin SM. Embryo number and periconceptional undernutrition in the sheep have differential effects on adrenal epigenotype, growth and development. Am. J. Physiol. Endocrinol. Metab. 2014;307(2):141-50.
11. Choi SH, Choi-Kwon S, Kim MS, Kim JS. Poor nutrition and alcohol consumption are related to high serum homocysteine level at poststroke. Nutr. Res. Pract. 2015;9(5):503-10.
12. Ingenbleek Y, Hardillier E, Jung L. Subclinical protein malnutrition is a determinant of huperhomocysteinemia. Nutrition. 2002;18(1):40-6.
13. Ingenbleek Y, Mc Cully KS. Vegetarianism produces subclinical malnutrition, hyperhomocysteinemia and atherogenesis. Nutrition. 2012;28(2):148-53.
14. Kalhan SC, Marczewski SE. Methionine, homocysteine, one carbon metabolism and fetal growth. Rev. Endocr. Metab. Disord. 2012;13(2):109-19.
15. Lee HS. Impact of maternal diet on the epigenome during in utero life and the developmental programming of diseases in childhood and adulthood. Nutrients. 2015;7(11):9492-507.
16. Parimi PS, Cripe-Mamie C, Kalhan SC. Metabolic responses to protein restriction during pregnancy in rat and translation initiation factors in the mother and fetus. Pediatr. Res. 2004;56(3):423-31.
17. Morrison CD, Laeger T. Protein-dependent regulation of feeding and metabolism. Trends Endocrinol Metab. 2015;26(5):256-62.
18. Lillycrop KA, Phillips ES, Jackson AA. Dietary protein restriction of pregnant rats induces and folic acid supplementation prevents epigenetic modification of hepatic gene expression in the offspring. J. Nutr. 2005;135:1382-6.
19. Zabuga OG, Ahaladze NG, Vajserman AM. Metabolicheskoe programmirovanie: teoreticheskie koncepcii i ehksperimental’nye dokazatel’stva. Uspekhi gerontologii. 2013;26(2):212-23. [in Russiаn].
20. Berezovskyi VYa, Yanko RV, Litovka IH. Vplyv alimentarnoi depryvatsii na fiziolohichnu reheneratsiiu parenkhimy pechinky molodykh i doroslykh shchuriv. Fiziol. zhurn. 2008;54(6):66-71. [in Ukrainian].
21. Nikolaieva OV, Kovaltsova MV, Yevtushenko TH, vynakhidnyky; Kharkivskyi natsionalnyi medychnyi universytet, patentovlasnyk. Sposib modeliuvannia alimentarnoi nedostatnosti. Patent Ukainy № 81453. 2013 Cher 25. [in Ukrainian].
22. Avtandilov GG. Medicinskaya morfometriya. M.: Medicina; 1990. 384 s. [in Russiаn].
23. Kamyshnikov VS. Metody klinicheskih laboratornyh issledovanij. Moskva: «Medipress – inform»; 2016. 736 s. [in Russiаn]. 

«Bulletin of problems biology and medicine» Issue 4 Part 2 (147), 2018 year, 151-156 pages, index UDK 616. 36 – 091. 8 – 092. 9: 613. 24

10.29254/2077-4214-2018-4-2-147-151-156