Pantus A. V., Rozhko M. M., Kovalchuk N. E., Yarmoshuk I. R.

METHODS AND METHODOLOGIES

Scentific article

Abstract. Today, the problem of restoration or replacement of tissues and organs using bio-scaffolds remains an urgent issue. When creating a tissue-engineered implant, it is important to provide matrix materials with a complex three-dimensional fibrous scaffold structure (non-woven scaffold) with a high ratio of surface area to total volume, imitating the intercellular tissue matrix. The currently existing promising method for synthesizing a fibrous matrix is electrospinning, which is energy-intensive and, accordingly, expensive. Currently, the development of a cheaper and cleaner method for the synthesis of fibrous matrices remains relevant. The aim of our study was to assess the nature of the structure of synthesized micro-flocks at various polymer concentrations. To carry out the research, we used a fibrous matrix obtained by our developed rotational synthesis from granules of 100% pure polycaprolactone (PCL) and polyllactide (PLLA). The resulting fibrous matrix was analyzed under a Leica DME light microscope. So, according to the results of the analysis of the thickness and microstructure of microfibers of thermoplastic biopolymers PCL and PLLA, it can be concluded that at very low initial concentrations of polymers in the sucrose melt, microfibers with the smallest transverse diameter prevailed in the fibrous matrices, but in the form of thin welded and twisted fragmented threads with available globules on their surface. This effect can be explained by the fact that during the synthesis of microfibers at low polymer concentrations and uneven stretching leads to twisting, adhesion and soldering to each other. With an increase in the polymer concentration, its volume in the sucrose melt, on the contrary, made it possible to fully stretch the fiber, which led to an increase in the number of microfibers more organized in the form of thin and thick microfibers in the matrix.

: biopolymer, matrix, microfiber, sucrose.

1. Sharma A, Faubion WA, Dietz AB. Regenerative Materials for Surgical Reconstruction: Current Spectrum of Materials and a Proposed Method for Classification. Mayo Clin. Proc. 2019;10(94):2099-2116.
2. Sevostyanova VV, Golovkin AS, Filipev DE, Glushkova TV, Borisov VV, Burago AYu, et al. Vyibor optimalnyih parametrov elektrospinninga dlya izgotovleniya sosudistogo grafta malogo diametra iz polikaprolaktona. Fundamental’nyye issledovaniya. 2014;10(1):180-184. [in Russian].
3. Conway J, Jacquemet G. Cell matrix adhesion in cell migration. Essays in Biochemistry. 2019;5(63):2012-9.
4. EUCAST. Clinical breakpoints – bacteria v 9.0 [Internet]; 2019 [ updated 2019 Jan 1]. Available from: http://www.eucast.org/clinical_ breakpoints/.
5. Markakis K, Faris AR, Sharaf H, Barzo Faris, Rees S, Bowling FL. Local Antibiotic Delivery Systems: Current and Future Applications for Diabetic Foot Infections. Int. J. Lower Extremity Wounds. 2018;1(17):14-21.
6. Marson BA, Deshmukh SR, Grindlay DJC, Ollivere BJ, Scammell BE. A systematic review of local antibiotic devices used to improve wound healing following the surgical management of foot infections in diabetics. Bone Joint Journal. 2008;11(100):1409-1415.
7. Garvin K, Feschuk C. Polylactide-polyglycolide antibiotic implants. Clin. Orthop. Relat. Res. 2005;437:105-110
8. Suchý T, Šupová M, Klapková E, Adamkova V, Zavora J, Zaloudkova M, et. al. The release kinetics, antimicrobial activity and cytocompatibility of differently prepared collagen/hydroxyapatite/vancomycin layers: Microstructure vs. Nanostructure. Eur. J. Pharm. Sci. 2017;100:219-229.
9. Nishimura J, Nakajima K, Souma Y, Takahashi T, Ikeguchi N, Takenaka R, et. al. The possibility of using fibrin-based collagen as an antibiotic delivery system. Surg. Today. 2013;2(43):185-190.
10. Costa Almeida CE, Reis L, Carvalho L, Costa Almeida CM. Collagen implant with gentamicin sulphate reduces surgical site infection in vascular surgery: A prospective cohort study. Int. J. Surg. 2014;10(12):1100-1104.
11. Rapetto F, Bruno VD, Guida G, Marsico R, Chivasso P, Zebele C. Gentamicin-Impregnated Collagen Sponge: Effectiveness in Preventing Sternal Wound Infection in High-Risk Cardiac Surgery. Drug Target Insights. 2016;1(10):9-13.

«Bulletin of problems biology and medicine» Issue 1 (159), 2021 year, 174-176 pages, index UDK 616-089.843+615.461+576.31

10.29254/2077-4214-2021-1-159-174-176