Miroshnik D. B., Shckorbatov Y. G.

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

At present it is not possible to achieve a complete efficacy in the treatment of doxorubicin because medication is administered before the manifestation of side effects. For this reason, many studies are being conducted to develop techniques to improve the effectiveness of doxorubicin treatment. It was proposed, for example, to use the combined effect of magnetic or electromagnetic fields and doxorubicin to reduce toxicological load on the human organism. The purpose of the present study was to investigate the combined effect of a static and rotating magnetic fields and doxorubicin on human buccal epithelial cells, and to determine the difference in the influence of a static magnetic field and a rotating one. The combined effect of doxorubicin and magnetic field of induction of 16.7 mT on the viability of isolated buccal epithelial cells of three donors was investigated. The magnetic field was used as a static if magnet was unmovable, and rotating magnetic field if magnet turned around at a speed of 500 rpm. Doxorubicin at a concentration of 2 μg/ml added to cell culture medium for 2 hours has been shown to increase the decrease of cell viability and to the heterochromatization of chromatin in cell nuclei. The influence of a static magnetic field of 16.7 mT for 10 minutes does not lead to a reliable change of the studied parameters (neither increase of heterochromatin granules nor increase of membrane permeability to ethidium bromide). The influence of the rotating magnetic field at the same time as doxorubicin leads to a decrease in the number of heterochromatin granules in the nuclei of the cells of all three donors and in a decrease in the permeability of the membranes to the ethidium bromide in the two donors compared to the variant effect of the doxorubicin alone. This indicates a protective effect of magnetic field in the case of using in combination with doxorubicin. The cause of the effect in the experiment is assumed to be connected with hormesis effect.

cell viability, heterochromatin, cell membrane, membrane permeability, magnetic field.

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«Bulletin of problems biology and medicine» Issue 4 Part 2 (154), 2019 year, 157-161 pages, index UDK 576.32/.36:612.014

10.29254/2077-4214-2019-4-2-154-157-161