Gordienko G. R., Sikora V. Z.
IMMUNOHISTOCHEMICAL FEATURES OF THE RAT STOMACH DURING INTERMITTENT FASTING
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About the author:
Gordienko G. R., Sikora V. Z.
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PROSPECTS FOR THE DEVELOPMENT OF MORPHOLOGICAL AND CLINICAL RESEARCH
Type of article:
Scientific article
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Intermittent fasting is a popular dietary approach with a mixed reputation. Some scientists insist on its benefits for the body, while others warn of possible dangers. In this study, we focused on the immunohistochemi- cal characterisation of the stomach state of rats during intermittent fasting (alternate-day fasting – “feeding day” : “fasting day”). The study design involved the formation of one control and three study groups, each comprising 18 animals, which were intermittently fasted for one, two, and four weeks.. Immunohistochemical studies were performed at the Center for Pathomorphological Research of Sumy State University according to a standard protocol using monoclonal antibodies against vimentin (clone V9) and neuron-specific enolase (clone 22C9) from Leica Biosystems (Germany). A significant increase in the expression of vimentin in the study groups compared to the control (p˂0.001) and neuron-specific enolase (p˂0.01 – after one week, p˂0.05 – after two weeks, p˂0.001 – after four weeks of the experiment) was found. A persistent long-term increase in vimentin expression may indicate exhaustion of adaptation processes and chronic damage to gastric tissue. A gradual, stable increase in the expression of neuron-specific enolase may indicate an increase in local neuronal activity, which, under conditions of prolonged starvation, can lead to overstrain of the local nervous system, hyperplasia of neuroendocrine glands, increased secretion of hydrochloric acid, and chronic damage to the stomach. This may indicate a negative effect of alternate-day fasting on the stomach condition of rats and the feasibility of using these markers to monitor stomach condition during intermittent fasting.
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Bibliography:
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Publication of the article:
«Bulletin of problems biology and medicine», 2025 Issue 4, 179, addition, 26-28 pages, index UDC 616-091.8
