Lutsyk S. A., Yashchenko A. М.

MORPHOLOGY

Scentific article

Using WGA lectin the morphogenesis of rat adrenal glands in physiological conditions and under maternal hypo- and hyperthyroidism was investigated. On the day 20th of prenatal ontogenesis in the central part of control group animals adrenal glands were identified islets of cells with large basophilic nuclei and light cytoplasm, which were considered as chromaffine cells precursors of adrenal medulla. Endothelium of microvascular bed showed marked affinity for WGA lectin, encompassing exposure of DGlcNAc and NeuNAc carbohydrate determinants. In progeny developed under maternal hypothyroidism it was detected the enlargement of vascular bed of the adrenal medulla accompanied with the lack of chromaffin cells, which apparently indicate a delay in the adrenal gland morphogenesis. On the postnatal day 1st adrenal medulla consisted of elongated clusters of chromaffin cells, subdivided with the blood vessels, whereas on the 10th postnatal day in all experimental groups medulla was clearly separated from cortex. On the background of complete lectin areactivity of adrenocorticocytes, chromaffine cells demonstrated the accumulation of WGA lectin receptor sites: the degree of exposure was higher in offspring that developed under hypo- and hyperthyroidism, which could be regarded as certain violation of adrenal morphogenesis. Lectin reactivity of endothelial cells in zona glomerulosa and zona fasciculata reduced during postnatal development, while endothelium of zona reticularis, in parts, preserved affinity for it. On the postnatal day 40th and in the adult rats adrenal glands medulla consisted of two subpopulations of chromaffine cells: strong WGA-positive epinephrocytes, and less reactive norepinephrocytes. Maternal hyperthyroidism induced hypertrophy of adrenal gland cortical part. The obtained data makes it possible recommendation of WGA lectin as a selective histochemical marker of chromaffine cells with the possibility of differential detection of epinephrocytes and norepinephrocytes. Maternal hypothyroidism induced delay of adrenal gland maturation on early stages of postnatal development, whereas thickening of the adrenal cortex was the most characteristic sign for maternal hyperthyroidism.

rat adrenal gland, postnatal morphogenesis, maternal hypo- and hyperthyroidism, lectin histochemistry

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«Bulletin of problems biology and medicine» Issue 2 (144), 2018 year, 325-329 pages, index UDK 612.451-018.1:(616.441-008.64+616.441-008.61):581.162.1-092.4/.9

10.29254/2077-4214-2018-2-144-325-329