Havrysh R., Lukavetskiy O., Havrysh Y.

MORPHOLOGY

Scentific article

Aim. To study effect of gastric plication on the structure of the pancreas in rats with streptozotocin induced diabetes mellitus. The object and methods of research. Experimental part of scientific work was carried out on adult white rats. For the experiment 42 rats were involved. All animals were divided into 4 groups: Group I – control group (n-8) – rats without streptozotocin administration and did not undergo a gastric plication surgery; Group II (n-8) – rats that did not receive streptozotocin, but underwent gastric plication surgery; Group III (n-8) – rats with streptozotocin induced diabetes mellitus, without gastric plication; Group IV (n-18) – rats with streptozotocin induced diabetes mellitus, which has underwent gastric plication. Diabetes mellitus was inducted by streptozotocin from Appli Chem (USA). Intraperitoneal administration of streptozotocin was performed for four consecutive days in a single dose of 40 mg/kg body weight. The experiment lasted for 6 months. Research results and their discussion. Only 30 laboratory rats (71.5%) survived to the end of experiment. The mortality rate: 0 animals died in the first control group; 1 died (12.5%) in the second group (only surgery); 4 (50%) died among the animals of the third group, (rats with DM and witout surgery) and 7 animals (38.9%) died from of the fourth group (DM+surgery). At the end of experiment, we did not make a significant difference in blood glucose values in animals of groups I and II. In group III, blood glucose levels were 454% higher compared with control group and 211% higher compared to group IV of animals undergoing gastroplasty. In animals of group IV, the mean GL score was 11.86 ± 2.47 mmol/l, which was 215% higher compared with control group I. At the end of experiment, we took the pancreatic tissue for histological examination. In each animal, we performed several sections of the pancreas and determined the mean perimeter of the Langerhans islands and their area. Conclusions. We noted significantly lower blood glucose levels (50%, p <0.05) at the end of experiment in rats that underwent gastric plication compared to animals only with streptozotocin induced diabetes mellitus. The average perimeter and area of Langerhans islands was significantly bigger in rats that underwent gastric plication compared to animals only with streptozotocin induced diabetes mellitus. Our data give big insight for deeply study of gastric plication effects on the course of diabetes mellitus.

gastric plication, bariatric surgery, type 2 diabetes, streptozotocin induced diabetes mellitus, rat

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«Bulletin of problems biology and medicine» Issue 3 (145), 2018 year, 270-275 pages, index UDK 616.33-089.848-06:616.379-008.65]-02:616.37-018-092.9

10.29254/2077-4214-2018-3-145-270-275