Rogulska O. Yu., Bozhok G. A., Trufanova N. A., Revenko O. B., Pakhomov O. V., Cherkashina D. V., Petrenko O. Yu.

FORMATION OF SPHEROIDS FROM HUMAN ADIPOSE TISSUE-DERIVED MESENCHYMAL STEM CELLS BY THE HANGING DROP METHOD

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About the author:

Rogulska O. Yu., Bozhok G. A., Trufanova N. A., Revenko O. B., Pakhomov O. V., Cherkashina D. V., Petrenko O. Yu.

Heading:

NANOMEDICINE AND NANOTECHNOLOGY

Type of article:

Scientific article

Annotation:

Spheroids from mesenchymal stromal cells (MSCs) of human adipose tissue are a promising source of autologous stem cells that are easy to obtain, cryopreserve, store, and use as needed. The present study aimed to determine the optimal modes of obtaining spheroids from human adipose tissue MSCs by the hanging drop method. The study used MSCs from 3-4 passages of human adipose tissue at seeding concentrations of 1, 3, 5, 7 and 10×103 cells/drop. MSCs were cultivated in a 30 μL hanging drop at 37°C and 5% CO2 for 72 hours. The morphological properties, size, number of spheroids formed, and viability of cells in their composition were evaluated depending on the seeding concentration of cells. The results showed that at seeding concentrations of 1, 3, 5, and 7×103 cells/drop, the process of spheroid formation was completed within 72 hours of cultivation, while at the seeding concentration of 10×103 cells/drop – it was completed within 48 hours. The average diameter of the formed spheroid was positively correlated with the initial concentration of MSCs, with about 90% of cells in spheroids formed at concentrations of 1, 3 and 5×103 cells/drop remaining viable, while at seeding concentrations of 7 and 10×103 cells/drop there was a significant decrease in cell viability. Based on a comprehensive analysis of the data obtained, the optimal conditions for spheroid formation from human adipose tissue MSCs by the hanging drop method are using a seeding concentration of 3-5×103 cells/drop and a culture period of 72 hours.

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Bibliography:

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Publication of the article:

«Bulletin of problems biology and medicine», 2023 Issue 3, 170, 423-432 pages, index UDC 576:602

DOI:

10.29254/2077-4214-2023-3-170-423-432