Poladych I. V.

FIBROBLAST GROWTH FACTOR 23 IN PREGNANT RATS WITH DIFFERENT VITAMIN D3 STATUS

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About the author:

Poladych I. V.

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CLINICAL AND EXPERIMENTAL MEDICINE

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Scientific article

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Vitamin D₃ deficiency during pregnancy is associated with impaired calcium-phosphorus homeostasis and the development of gestational complications. Fibroblast growth factor 23 (FGF23), a peptide hormone that regulates phosphate metabolism and active vitamin D3 synthesis, may play a critical role in these processes. However, its systemic and placental activity during pregnancy remains poorly understood. The aim is to investigate FGF23 levels in the serum and placenta of pregnant rats with different vitamin D3 statuses and to analyze its associations with key biomarkers of mineral metabolism. This study included 36 pregnant Wistar rats divided into three groups: control, vitamin D₃-deficient, and D₃-corrected with cholecalciferol. Serum and placental levels of 25(OH)D₃ and FGF23 were measured using ELISA. Statistical analysis was performed with ANOVA and correlation tests. In rats with vitamin D₃ deficiency, serum FGF23 levels were significantly increased (471.7 ± 20.4 pg/mL) com- pared to the control group (211.5 ± 15.82 pg/mL; p<0.001). Correction with cholecalciferol reduced the level to 173.4 ± 12.5 pg/mL (p<0.001). Placental FGF23 showed a slight increase in the deficiency group, though differences were not statistically significant. FGF23 levels negatively correlated with 25(OH)D3 (r= -0.75) and positively with PTH (r=0.62). FGF23 responds to vitamin D₃ deficiency by increasing systemic levels and may contribute to mineral imbalance during pregnancy. Its normalization after D₃ correction suggests potential as a biomarker of gestational disturbances linked to mineral metabolism.

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Publication of the article:

«Bulletin of problems biology and medicine», 2025 Issue 3,178, 213-220 pages, index UDC 612.015.3:616-092.9-008.6:577.17](083.1)

DOI:

10.29254/2077-4214-2025-3-178-213-220