Fik V. B., Dmytriv G. M., Fedechko Y. M., Paltov Ye. V.
Oral microbiome is a well-balanced dynamic ecosystem and is an important and complex bacterial community in the body. The study was performed on 20 white outbred adult male rats, weighing 160 grams, aged 4,5 months. Before the experiment, a thorough examination of the animals was performed, visually paying attention to the color and moisture of the mucous membrane of the oral cavity. Collection of material was carried out on the first, fourteenth and twenty-eighth days. The material for the study was the microbiota of different parts of the oral cavity of rats, namely, from microbiotopes of the tooth surface in the gingival margin, mucous membrane of oral vestibule and mucous membrane of oral cavity proper. In microscopic examination the smear material was applied on a glass slide, fixed over a burner flame and Gram stained. In bacteriological studies, аs a result of counting the total number of sprouted colonies, a quantitative indicator in colony-forming units was obtained per 1 ml (CFU/ml). For further statistical analysis, the obtained data were tested for normality by calculating the asymmetry and excess coefficients and the results of the Shapiro-Wilk test (p<0.05). The data were presented as M±SD, where M is the mean, and SD is the standard deviation. Microscopic examination of smears from microbiotopes of the oral cavity revealed that the microbiota was the same in all intact animals. Studies conducted at different times of the experiment (1, 14 and 28 days) indicated the dominance of gram-positive microorganisms. The smears showed elongated filamentous bacteria – Leptotrix (genus Lactobacillus). Gram-positive coccal microbiota was visualized as separate clusters. When comparing the quantitative characteristics of the streptococcal microbiota of the microbiotope of the tooth surface in the gingival margin for twenty-eight days, it was noted that the number of nonhemolytic and α-hemolytic streptococci did not change significantly during the experiment, in contrast to other studied microbiotopes of oral intact animals. At the same time, in comparison with the previous term (14 days) of the experiment, the number of α-hemolytic streptococci in the microbiotope of mucous membrane of oral vestibule increased significantly relative to the quantitative composition of these microorganisms in the microbiotoре of mucous membrane of oral cavity proper. Quantitative indicators of other species of detected microorganisms (coagulase-negative staphylococci, Еnterococci, Escherichia coli, grampositive non-spore forming rods and spore forming rods) did not differ significantly in the microbiotopes of the oral cavity of rats, formed on the twenty-eighth day of the experiment. This indicated the stabilization of the microbiocenosis of the oral cavity in the quarantine of intact animals in the experimental conditions.
oral cavity, microbiotopes, rats, bacterial groups.
«Bulletin of problems biology and medicine» Issue 2 Part 2 (165), 2022 year, 17-21 pages, index UDK 611.31.018:616-008.87
