Lyubovich О. Ye., Klishch I. M.

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

Hypothyroidism is one of the most common pathologies of the endocrine system, caused by deficiency of thyroid hormones or a decrease in their biological effect at the tissue level. The prevalence of hypothyroidism in the general population reaches 3.7 %, depending on age, sex, and level of iodine intake. The aim of our study was to study the features of the cytokine profile of blood in rats in the dynamics of immobilization stress on the background of hypothyroidism. The study was conducted on 84 sexually mature white male Wistar rats. The concentrations of TNF-α, IL-1β and IL-6 were studied by a solid-phase enzyme-linked immunoabsorbent assay (ELISA) using RayBio reagents produced by RayBiotech (USA). It has been established that modeling of immobilization stress in rats is accompanied by hyperproduction of proinflammatory cytokines, which plays an important role in the initiation of the pathological process and the launch of metabolic cascade responses. Due to the ratio of IL-1β to TNF-α, the predominance of the inflammatory component in the anxiety stage, adaptation to stress after 48 hours in the stage of resistance, followed by progression in the stage of exhaustion has been established, which was accompanied by a significant increase in the index of destruction. In animals with deficiency of iodine-containing hormones of the thyroid gland, the simulation of immobilization stress is also accompanied by hyperproduction of proinflammatory cytokines. Due to the ratio of IL 1β to TNF-α the predominance of destructive processes at the anxiety stage with vs euthyroid rats, adaptation to stress after 48 hours at the resistance stage with the subsequent prevalence of the inflammatory component at the depletion stage was determined.

immobilization stress, hypothyroidism, cytokines, experiment

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«Bulletin of problems biology and medicine» Issue 1 Part 1 (148), 2019 year, 140-144 pages, index UDK 615.15-097-02:612.766.2-06:616.441-008.64]-092.9

10.29254/2077-4214-2019-1-1-148-140-144