Kylymniuk L. O., Kovalchuk V. M., Hryhorovska A. V., Okaievych O. A., Matsipura M. M.

MORPHOLOGY

Scentific article

Aim: to establish the morphological features of healing process of fracture with unstable fixation under the influence of extracorporeal shockwave therapy. Object and methods. The study was performed on 40 healthy male rats weighing 332,53±14,47 g. The osteotomy of diaphysis of the femoral bone with followed intramedullary fixation was performed under combined anesthesia. Two groups were selected – control (n=20) and experimental (n=20). In the experimental group, the bone healing process were stimulated by using extracorporeal shockwave therapy. The whole course of treatment consists of 4 procedures with 7 days intervals. The extracorporeal shockwave therapy was performed by apparatus Swiss Dolor Clast (Electro Medical Systems, Nyon, Switzerland). The animals were taken out of the experimental on 14, 21, 28, 35 days of postoperative period, for 5 rats from each group. Specific volumes of bone (BV/TV) and connective tissue (CV/TV) were determined by linear integration method. The probability of an error-free prediction was set at p≤0.05. For statistical data processing the Statistica 10 program was used. Results. In both groups, BV/TV continuously increased to 35 days, by 1.9 in the experimental group, in the control group – by 1.28. The formation of bone tissue in the experimental group developed 1.48 faster. In the experimental group, marked a rapid decrease in CV/TV by 5.46 to 35 days, in the control group, these figure increased 2.31. In the experimental group, a significant increase in BV/TV (r =+0.6323) and a significant decrease in CV/TV (r=0.6437) during the study was established. There was no significant difference in BV/TV in the control group, while a significant increase in CV/TV was established (r=+0.7911). Conclusions. In the experimental group, a positive tendency was observed in the formation of bone tissue and negative in the development of the connective tissue. In the control group, positive dynamics of the formation of connective tissue was observed.

fracture healing complication, extracorporeal schockwave therapy, stimulation of reparative osteogenesis, reparative osteogenesis, morphological features of fracture healing

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«Bulletin of problems biology and medicine» Issue 2 (144), 2018 year, 310-315 pages, index UDK 616.718.4-001.5-089.85-018.4:615.849.112:534.222.2:621.374.001.6

10.29254/2077-4214-2018-2-144-310-315