Tymchuk K. M., Kryzhanovskyi D. G., Trushenko O. S., Shevchenko I. F., Zherzhova T. A., Davydenko I. V., Konovalova O. S.
In recent years, in connection with the intensive development of industry, there has been an increase in the content of heavy metals in the environment, which has a detrimental effect on the human body, animals and plants. Cd enters the body of humans and animals mainly in the following ways: through the gastrointestinal tract (alimentary), respiratory organs (inhalation) and the surface of the body (transcutaneous), from where this element is absorbed into the blood, and, having high cumulative properties, up to 20% accumulates in the small intestine. Cadmium (Cd) is the most widespread pollutant of the environment and has a toxic effect in small concentrations, has an acute and chronic effect on health. It accumulates throughout life. The main role in terms of the natural biological barrier is played by the intestinal epithelium, which reflects the body’s ability to resist the action of various exotoxicants, including cadmium. The purpose of the study is to determine the effect of low doses of cadmium (2,0 mg/kg) and copper (0,1 mg/ kg) on the general course of embryogenesis in rats with combined administration throughout the entire period of pregnancy of white rats. Females with a dated gestation period were obtained for the research, and the estrous cycle of the females was studied by the method of vaginal smears. At the stage of estrus and proestrus, they were mated with intact males according to the 2:1 scheme. The solutions were administered through a tube, intragastrically, daily (once a day) from the first day of pregnancy: Group I – control, Group II – administration of cadmium chloride solution at a dose of 2,0 mg/kg – group of isolated administration of cadmium, and Group III – group of combined administration cadmium chloride, at a dose of 2,0 mg/kg, together with copper succinate at a dose of 0,1 mg/kg. The cadmium chloride solution was of ionic form, and the copper succinate solution was of nanoaquachelate form. On the 13th and 19th days of pregnancy, females were slaughtered. The analysis of the obtained data indicates a pronounced embryotoxic effect of cadmium chloride on the processes of embryogenesis, which is a significant increase in the indicators of total embryonic mortality, preimplantation and post-implantation mortality compared to the control group, at all studied terms of embryogenesis.
cadmium,copper,embryogenesis,embryos,small intestine.
«Bulletin of problems biology and medicine» Issue 4 (167), 2022 year, 114-118 pages, index UDK 612.3:591.39:661.852:661.782-092.9
