Pitko V. A., Loginova O. A., Novikova O.А., Sinilo N. N., Cherkashina J. O., Pavlov I. V., Gaviley O. V.

METHODS AND METHODOLOGIES

Scentific article

In recent years, there has been an increase in the trend towards embryo transfer in cryocycles. The growing popularity is primarily due to the reduction of the impact and consequences of hormonal stimulation of the ovaries on the body of women. Cryopreservation allows the use of high-quality material in assisted reproductive technologies, without resorting to repeated hormonal stimulation, and can also be used for prenatal genetic diagnosis, which not only prevents the birth of children with genetic diseases, but in many cases increases the effectiveness of treatment in vitro fertilization programs. The quality of embryos is not the only factor influencing the successful implantation, no less important component are the factors caused by the condition of the uterus. The aim of the work was to evaluate the effectiveness of using the method of double embryo transfers in cryocycles. Two groups of patients aged from 26 to 45 years were selected for the study, in the treatment of which double and conventional (one embryo transfer) cryopreservations were used during 2018-2019. The embryos were cryopreserved at the blastocyst stage (5 days of development) by vitrification. The number of embryos transferred to the uterine cavity in conventional embryo transfer was 1 or 2, sometimes 3 embryos (with the consent of patients), in double – twice 1 embryo. The results between the groups were compared for the presence or absence of pregnancy, based on the results of tests to determine human chorionic gonadotropin (HCG) and the number of clinical pregnancies. The evaluation of the statistical hypothesis was performed using the criterion (Chi-square) p=0.02, with n=2. The analysis showed that double embryo transfers make it possible to increase the effectiveness of cryocycles and reduce the incidence of multiple pregnancies. The use of this method made it possible to increase the frequency of pregnancies in patients of older and middle reproductive age.

embryos, vitrification, implantation, “double” cryo-transfers, “implantation window” endometrium, stimulation.

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«Bulletin of problems biology and medicine» Issue 3 (161), 2021 year, 225-228 pages, index UDK 612.646-089.6:57.086.13

10.29254/2077-4214-2021-3-161-225-228