Dybkova S. M., Rieznichenko L. S., Gruzina T. G.

ELECTROACTIVE COMPLEXES OF GLUCOSE OXIDASE WITH GOLD NANOPARTICLES FOR THE DEVELOPMENT OF ENZYMATIC SENSOR ELEMENTS OF ELECTROCHEMICAL BIOSENSORS

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About the author:

Dybkova S. M., Rieznichenko L. S., Gruzina T. G.

Heading:

NANOMEDICINE AND NANOTECHNOLOGY

Type of article:

Scientific article

Annotation:

Gold nanoparticles can be effectively used to enhance the direct electron transfer between enzymes and electrodes, increase the catalytic activity of enzymes, and improve the immobilization of enzymes on the electrode, which significantly increases the sensitivity of enzyme sensors. Therefore, the creation of effective enzyme complexes with gold nanoparticles is an urgent task. The aim of this work was to study the interaction of the glucose oxidase enzyme with gold nanoparticles by investigating the formation of their electroactive complexes. Spectroscopic, electron microscopic, electrophoretic and biochemical methods were used in the work. The contact interaction of glucose oxidase with gold nanoparticles of different sizes was investigated and the ability of such nanoparticles to form electroactive complexes with glucose oxidase at certain concentrations was established. The stimulation of the catalytic activity of glucose oxidase in such electroactive complexes was established. The effectiveness of the study of electroactive complexes of enzymes with metal nanoparticles by agarose gel electrophoresis for the development of sensor elements of enzyme electrochemical biosensors is shown.

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Bibliography:

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Publication of the article:

«Bulletin of problems biology and medicine», 2024 Issue 3, 174, 333-340 pages, index UDC 543.06 + 577.15 + 543.555

DOI:

10.29254/2077-4214-2024-3-174-333-340