EFFECT OF N-CHLOROTAURINE ON BIOFILMS OF PATHOGENS ASSOCIATED WITH COMPLICATIONS OF DENTAL IMPLANTATION

  2. 2023
  3. EFFECT OF N-CHLOROTAURINE ON BIOFILMS OF PATHOGENS ASSOCIATED WITH COMPLICATIONS OF DENTAL IMPLANTATION

Efimenko A. O., Stepanskyi O. D., Bogomolny L. O., Ishchenko O. V.

EFFECT OF N-CHLOROTAURINE ON BIOFILMS OF PATHOGENS ASSOCIATED WITH COMPLICATIONS OF DENTAL IMPLANTATION

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About the author:

Efimenko A. O., Stepanskyi O. D., Bogomolny L. O., Ishchenko O. V.

Heading:

DENTISTRY

Type of article:

Scientific article

Annotation:

Dental implants can be colonized by bacteria, leading to implant-related infections. N-chlorotaurine (NCT) is a promising remedy against biofilms. The present study aimed to investigate in vitro activity of NCT against long-term biofilms on titanium material used for implants. We studied the activity of 1% NCT against isolates obtained from patients with dental implantation failure. The study of biofilm-forming properties was performed in vitro on titanium disks, incubation was up to 8 weeks. To study the immediate effect of NCT on a biofilm, the XTT-reductase test was applied. The studied 1%-solution of NCT has demonstrated considerable bactericidal activity against all test bacteria already after 30 min of the study initiation. The best results were achieved for gram-positive bacteria, especially for Streptococcus spp. Results obtained in experiments with gram-negative bacteria also were promising. But microbial load below the detection limit was achieved later in comparison to gram-positive bacteria (p<0.01). On all weeks, after 30-min exposure, the bacterial load comprised 5.2 (0.3) log10 CFU/ml for Enterobacteriaceae and 5.2 (0.2) log10 CFU/ml for Acinetobacter spp. But after an additional 30 min of cultivation, the microbial load fell below the detection limit (p<0.01). The studied concentration of NCT also significantly reduced metabolism in microbial cells. High values for most of the studied isolates have been already achieved within the first 30 min of incubation. Subsequent incubation also promoted metabolic turnover; in comparison to 30 min after exposure, prolonged incubation within 60 min led to up to 85.0% reduction of metabolic activity (p<0.05). However, 3h exposure didn`t bring any statistically significant results. NCT in a millimolar concentration can efficiently limit the vital activity of microorganisms on biofilms produced in vitro.

Tags:

bacteria, bactericidal activity, biofilms, microorganisms, N-chlorotaurine

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Publication of the article:

«Bulletin of problems biology and medicine», 2023 Issue 2, 169, 432-437 pages, index UDC 579.242/.262:615.28:616.314-089.843-092-036

DOI:

10.29254/2077-4214-2023-2-169-432-437

2023 Issue 2, 169
bacteria bactericidal activity biofilms microorganisms N-chlorotaurine
29.06.2023
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