Among the current problems of modern dentistry, generalized periodontitis occupies one of the leading places. According to modern points of view, in response to prolonged persistence of the microflora in the periodontium a violation of microcirculation, tissue hypoxia, activation of free radical processes, disorganization of biomembranes with the release of biologically active substances (histamine, serotonin) develop. The aim of the research was to study the dynamics of biogenic amines (histamine, serotonin) in the blood of patients with generalized periodontitis accompanied by normo-, hyper- and hyporeactivity of the body after patch surgery. 216 people aged between 45 and 55 years with the diagnosis of generalized periodontitis of II, III degree were examined. Depending on the state of reactivity of the organism, the patients were divided into three groups: the first one included patients with normoreaction (132 people); the second group consisted of patients with hyperreaction (46 people); the third one contained patients with hyporeaction (38 people). According to the indications, all the patients underwent patch surgery. Blood sampling was performed after the surgery on the 1st, 2nd, 4th, 6th and 9th day. Biogenic amines were extracted from the blood with chloroform-budanol mixture, followed by the use of hydrochloric acid for purification and determined by fluorimetric method. Histamine forms a fluoridating complex with orthofolic aldehyde, serotonin – with ninhydrin. The obtained digital data was processed by variational-statistical methods of analysis on the IBM PC personal computer using standard Microsoft Excel software. The results of the study of the content of histamine and serotonin in the blood of patients with GP with normal reactivity of the body showed that their dynamics was biphasic with its maximum values on the 1st and 6th day after surgery. The first rise is closely related to the early manifestations of the initial phase of wound healing – traumatic inflammation. On the 6th day, the second peak of the content of biogenic amines in the blood was recorded. It should be taken into consideration, however, that it was less pronounced compared to that on the 1st day and over time coincided with the development of young connective tissue in the wound. By the end of the observations after further slow involution, they were recorded by restoring their balance. The results of the study of biologically active substances in the blood of patients with generalized periodontitis accompanied by the increased reactivity of the body showed that surgery contributed to a significant increase in their content in the blood. In this case, the amplitude of the increase was more pronounced in comparison with that which was typical for normoreactivity. In addition, in contrast to the first group, the dynamics of the indicators was monophasic with its maximum value on the 1st day, when the concentration of amines probably exceeded the initial value (p<0.05). In the following terms, the level of histamine and serotonin decreased according to the “lytic” type, remaining until the end of the observation above the initial and corresponding values, typical for the normoreactivity of the organism. In contrast to the first group, on the 9th day there was no normalization of these indicators and the restoration of histamine / serotonin balance in the blood. Hyporeaction was characterized by a later increase in the content of biologically active substances with a violation of the balance of histamine / serotonin in the blood. Therefore, it is necessary to continue studying the question of explaining the mechanism of different clinical course of the inflammatory-destructive process with changed and unchanged reactivity of the organism.
generalized periodontitis,reactivity of the organism,wound,histamine,serotonin.
«Bulletin of problems biology and medicine» Issue 3 (166), 2022 year, 547-556 pages, index UDK 616.314.17-008.1:612.017.1
