Dundiuk-Berezina S. I., Slobodian O. M.

CURRENT STATE OF THE NORMAL DEVELOPMENT OF THE DENTOFACIAL SYSTEM IN LABORATORY ANIMALS

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Dundiuk-Berezina S. I., Slobodian O. M.

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LITERATURE REVIEWS

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Scientific article

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As a result of the analysis of literary sources, it was found that the study and identification of the processes of formation of the dentofacial system of laboratory animals, in particular rats, allows for a deeper understanding of the evolutionary, structural and functional aspects of odontogenesis, to lay the basis for the development of bioengi neered mechanisms for tooth restoration, and also contributes to the creation of experimental models of diseases of the teeth and jaws (for example, caries, periodontitis, osteoporosis, disorders of tooth development). The importance of model organisms, for example rats, is the possibility of identifying the spatiotemporal dynamics of the formation of the dentofacial system during the period of embryogenesis. The classically accepted four-stage model of tooth de velopment is: placode stage, bud stage, cap stage and bell stage. In the dentofacial system of the rat, there are two morphologically and functionally distinct types of teeth: paired incisors and molars. The rat dentofacial system is a dynamic system that combines complex morphological organization with a high level of functional plasticity, making the rat a valuable model for studying not only normal development but also pathologies of the dentofacial region, as well as for testing tissue repair methods in experimental dentistry.

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Bibliography:

1. Munshi-South J, Garcia JA, Orton D, Phifer-Rixey M. The evolutionary history of wild and domestic brown rats (Rattus norvegicus). Sci ence. 2024;385:1292-7. DOI: 10.1126/science.adp1166.
2. Baker S, Ayers M, Beausoleil N, Belmain S, Berdoy M, Buckle A, et al. An assessment of animal welfare impacts in wild Norway rat (Rattus norvegicus) management. Animal Welfare. 2022;31(1):51-68. DOI: 10.7120/09627286. 31.1.005.
3. Guosheng W, Shengxi H, Chi J, Sun H, Ye H, Bhikoo C, et al. The differences of root morphology and root length between different types of impacted maxillary central incisors: a retrospective cone-beam computed tomography study. Am J Orthod Dentofacial Orthop. 2022;161(4):548-556. DOI: 10.1016/j.ajodo.2020.09.037.
4. Zhang Y, Gao J, Wang X, Wang J, Zhang X, Fang S, et al. Biomechanical factors in the open gingival embrasure region during the intrusion of mandibular incisors: a new model through finite element analysis. Front Bioeng Biotechnol. 2023;11:1149472. DOI: 10.3389/ fbioe.2023.1149472.
5. Mu Y, Tian R, Xiao L, Sun D, Zhang Z, Xu S, et al. Molecular Evolution of Tooth-Related Genes Provides New Insights into Dietary Adap tations of Mammals. J Mol Evol. 2021;89:458-71. DOI: 10.1007/s00239-021-10017-1.
6. Zhu Y, Gu L, Wang J, Han J, Gou J, Wu Z. DNA methylation profiling of CpG islands in trigeminal ganglion of rats with orofacial pain in duced by experimental tooth movement. BMC Oral Health. 2024;24:1474. DOI: 10.1186/s12903-024-05269-4.
7. Gao Y, Min Q, Li X, Liu L, Lv Y, Xu W, et al. Immune System Acts on Orthodontic Tooth Movement: Cellular and Molecular Mechanisms Biomed Res Int. 2022;2022:9668610. DOI: 10.1155/2022/9668610.
8. Feng Wang, Jiang W, Chen B, Li R. The Mechanism of MSX1 and PAX9 Implication in Tooth Development Based on the Weighted Gene Co-Expression Network Analysis. Russ J Dev Biol. 2021;52:187-98. DOI: 10.1134/S1062360421030085.
9. Luo SY, Wang S, Liu ZX, Bian Q, Wang XD. Six1 Regulates Mouse Incisor Development by Promoting Dlx1/2/5 Expression. Journal of Dental Research. 2024;103(10):1017-27. DOI: 10.1177/00220345241256286.
10. Fujikawa K, Nonaka N, Wang X, Shibata S, et al. An in situ hybridization study of syndecan family during the late stages of developing mouse molar tooth germ. Anat Sci In. 2022;97:358-68. DOI: 10.1007/s12565-022-00647-w.
11. Kim HJ, Irfan M, Sreekumar S, Phimon A, Kim S, Chung S. CRISPR-edited DPSCs constitutively expressing BDNF enhance dentin regen eration in injured teeth. eLife. 2025;14:RP105153 DOI: 10.7554/eLife.105153.3.
12. Jing J, Zhang M, Guo T, Pei F, Yang Y, Chai Y, et al. Rodent incisor as a model to study mesenchymal stem cells in tissue homeostasis and repair. Frontiers in dental medicine. 2022;3:1068494. DOI: 10.3389/fdmed.2022. 1068494.
13. Christensen MM, Hallikas O, Das Roy R, Väänänen V, Stenberg OE, et al. The developmental basis for scaling of mammalian tooth size. Proc. Natl. Acad. Sci. U.S.A. 2023;120(25):e2300374120. DOI: 10.1073/pnas. 2300374120.
14. Hermans F, Hemeryck L, Lambrichts I, Bronckaers A, Vankelecom H. Intertwined signaling pathways governing tooth development: a give and-take between canonical wnt and shh. Frontiers in cell and developmental biology. 2021;9:758203. DOI: 10.3389/fcell.2021.758203.
15. Hallikas O, Das Roy R, Christensen MM, Renvoise E, Sulic A-M, Jernvall J. System-level analyses of keystone genes required for mam malian tooth development. Molecular and developmental evolution. 2020;336(1):7-17. DOI: 10.1002/jez.b.23009.
16. Ali S, Farooq I, Khurram SA. An illustrated guide to oral histology. Wiley; 2021. Chapter 1, Tooth development; p. 1-13. DOI: 10.1002/97811 19669616.ch1.
17. Nevoránková P, Šulcová M, Kavková M, Zimčík D, Balková SM, Peléšková K, et al. Region-specific gene expression profiling of early mouse mandible uncovered SATB2 as a key molecule for teeth patterning. Sci Rep. 2024;14:18212. DOI: 10.1038/s41598-024-68016-3.
18. Vonk J, Shackelford TK, eds. Encyclopedia of Animal Cognition and Behavior. Cham: Springer; 2022. Chapter, Rodentia Morphology; p. 6088-6090. DOI: 10.1007/978-3-319-55065-7_751.
19. Pei SL, Chen RS, Chen MH. The crucial role of centrioles in tooth growth and development. Journal of the Formosan Medical association. 2025;124(3):271-7. DOI: 10.1016/j.jfma.2024.04.014.
20. Kliszcz J, Jekl V. Dentistry in Mice-like Rodents. Vet Clin North Am Exot Anim Pract. 2025;28(3):609-627. DOI: 10.1016/j.cvex.2025.06.001.
21. Hu S, Zheng C, Jiang P, Zhang Q, Liu Y, Dou L. 3D visualization of neurovascular networks in pulp-exposed rat molars using tissue clearing techniques. Odontology. 2025;113(4):1659-1666. DOI: 10.1007/s10266-025-01092-7.
22. Roth DM, Bayona F, Baddam P, Graf D. Craniofacial Development: Neural Crest in Molecular Embryology. Head and Neck Pathol. 2021;15:1-15. DOI: 10.1007/s12105-021-01301-z.
23. Wei Y, Huang D, Chen SY, Jiang Y, Yang K, Hu Z, et al. Measurement of the root surface area in rat molars through three-dimensional modeling. Archives of Oral Biology. 2025;170:106132. DOI: 10.1016/j.archoralbio. 2024.106132.
24. Tokavanich N, Wein MN, English JD, Ono N, Ono W. The role of wint signaling in postnatal tooth root development. Frontiers in dental medicine. 2021;2:769134. DOI: 10.3389/fdmed.2021.769134.
25. Vu TH, Takechi M, Shimizu M, Kitazawa T, Higashiyama H, Iwase A, et al. Dlx5-augmentation in neural crest cells reveals early development and differentiation potential of mouse apical head mesenchyme. Sci Rep. 2021;11:2092. DOI: 10.1038/s41598-021-81434-x.

Publication of the article:

«Bulletin of problems biology and medicine», 2026 Issue 1, 180, 73-81 pages, index UDC 611.314.018.019

DOI:

10.29254/2077-4214-2026-1-180-73-81