Peretyatko O. G., Yagnuk Y. А., Sklyar N. І., Krestetska S. L., Bolshakova G. М.
The museum of microorganisms of SI «ІМІ NАМS» has one of the largest and oldest in Europe collections of microorganisms, that exists from the times of the creation of the institute. The basic criterion of the quality of the methods of the long-term storing of microorganisms in the collections is a possibility to renew their vital activity and stable their biological properties. Purpose of the research. Estimation of effectiveness of the lyophilization as the method of their long-term storage. Checking vital activity and correspondence to the taxonomic position of the museum strains of Staphylococcus, which were stored in the collection of the museum of microorganisms of SI «ІМІ NАМS» for a long time. Object and the methods of research. In the research 25 lyophilized samples of the 23rd strains S. aureus, isolated in the period since 1930 to 1986 the years were used. Strains were stored in the lyophilized form from 17 to 62 the years. The recultivation of strains and the estimation of their vital activity were conducted through Koch’s method. Reindentification of microbial cultures was carried out with the use of API «Bio-Mеrieux», France (ID 32 STAPH). Statistical processing of obtained data was conducted with the use of methods of parametric statistics via Microsoft Excel 2007, STATISTICA 6.0. Results of the research and its consideration. 92,0 % of S. aureus renewed the vital activity. Affirmed, that a quantity of viable cells in the renewed strains varied from 103 to 109 CFU/ml, the average index of the survival of the investigated strains is 7,19 %. The correlate dependence between a quantity CFU and the period of storage of the sample was not detected (r=0). When examining morphological, tinctorial and cultural properties of the recultivated strains it was established that the investigated strains corresponded to the standard characteristics of Staphylococcus. The investigated colonies of Staphylococcus were represented by S-, R-, D-forms. The index ratios of dissociation of microbial populations with the use of recultivation of the strains of Staphylococcus being investigated varied from 10,0 % to 90,0 %. With the statistical processing of data, it was revealed that the correlation between the index ratios of dissociation and the period of storage of sample in the lyophilized state. According to the results, the research of biochemical properties of the investigated strains the correspondence 69,6 % of the samples of the primary specific identification to S. aureus was established. Remaining strains 30,4 %, according to their biochemical profile, related to the type Staphylococcus, but to others species. Conclusion. The lyophilization of bacteria is an effective method of the long-term storage of the strains of microorganisms in the collections. A colonial polymorphism was observed with the renewal of vital activity of the lyophilized samples of the museum strains of Staphylococcus. The dissociation degree of bacterial population with the use of recultivation was dependent on the duration of storage of the lyophilized culture. 69,6 % of the recultivated strains corresponded to biochemical profile S. aureus.
lyophilization, museum strains of S. aureus, vital activity, species identification.
«Bulletin of problems biology and medicine» Issue 4 Part 1 (153), 2019 year, 211-213 pages, index UDK 57.083.1:57.086.132:579.861.2:57.083.182
