Podzihun L. V., Maievskyi O. Ye.

CHANGES IN MORPHOMETRIC INDICATORS OF THE RAT SPLEEN THREE DAYS AFTER ADMINISTRATION OF LEIURUS MACROCTENUS SCORPION VENOM

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About the author:

Podzihun L. V., Maievskyi O. Ye.

Heading:

MORPHOLOGY

Type of article:

Scientific article

Annotation:

The spleen plays a key role in protecting the body, detoxifying it, and maintaining immune balance. Participation in modulating the immune response may include changes in its histological structure, both qualitative and quantita tive. Determining the morphofunctional changes in the spleen caused by toxins of animal origin is critically import ant, since a detailed understanding of the damage at the microscopic or submicroscopic levels remains unexplored to this day. The aim of the study was to determine the features of changes in the morphometric parameters of the spleen of rats within 3 days after the administration of the venom of the scorpion Leiurus macroctenus. Experimental studies were conducted on male rats, which were injected intramuscularly with 0.5 ml of the venom solution (28.8 mg/ml) (LD50 = 0.08 mg/kg). For microscopic examination, samples of the animals' spleens were taken. Quantitative analysis was performed on digital images of histological sections stained with eosin and hematoxylin, using the clas sical method. Images were obtained from histological preparations of 4 animals from the control and experimental groups, 8 images per group, using a light microscope at a magnification of x100; morphometry was performed in the Fiji/ImageJ program. One day after exposure to the venom of scorpions of the Leiurus macroctenus species on the spleen of rats, a decrease in the proportion of white pulp, an increase in the number of macrophages with lipofuscin pigment inclusions, and a decrease in the thickness of the capsule in relation to the early observation periods are ob served. Three days later, an increase in the proportion of white pulp, a further decrease in the thickness of the spleen capsule, and an increase in the number of macrophages with brown pigment inclusions are observed.

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Bibliography:

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Publication of the article:

«Bulletin of problems biology and medicine», 2026 Issue 1, 180, 433-441 pages, index UDC 61:612.1:615.9.616.4:616-099

DOI:

10.29254/2077-4214-2026-1-180-433-441