Shmyr S. M., Klishch I. M.

BLOOD PROTEINASE-INHIBITOR SYSTEM IN RABBITS WITH MECHANICAL NONPENETRATING CORNEAL INJURY AND ITS CORRECTION WITH THE USE OF XENOCORNEAL STROMA RECEIVED BY THE DECELLULARIZATION METHOD

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About the author:

Shmyr S. M., Klishch I. M.

Heading:

CLINICAL AND EXPERIMENTAL MEDICINE

Type of article:

Scientific article

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Indicators of the proteinase-inhibitor system in rabbits under non-penetrating mechanical corneal injury and its correction using porcine xenocornea stroma obtained by decellularization were studied. The total proteolytic activity of blood plasma (TPA) was determined by the lysis of azoalbumin (degradation of low molecular weight proteins), azocasein (degradation of high molecular weight proteins) and azocoll (collagenolysis). To determine the state of the antiprotease system in the blood serum, the content of α1 protease inhibitor (α1-PI) and α2-macroglobulin (α2-MG) was determined. The proteolysis index (PI), which reflects the intensity or "controllability" of proteolytic processes, was also calculated as the ratio of TRA to the total inhibitory capacity (the sum of α1-IP and α2-MG activity). It was found that the simulation of mechanical non-penetrating corneal injury in the early stages of observation (3rd - 7th day) was accompanied by an increase in blood proteolytic activity, which was expressed in a significant increase in the concentration of azoalbumin, azocasein and azocoll in the blood serum and an increase in the concentration of antiprotease protection factors - α1-IP and α2-MG, indicating a compensatory increase in antiproteolytic potential to restrain the development of destruction. In later periods (14th - 28th day), the activity of proteolysis processes decreased, but by the 28th day, it significantly exceeded the level of animals without pathological process. At the same time, antiprotease protection decreased, which led to the preservation of significantly higher proteolysis index values. Surgical correction of mechanical trauma using the equivalent of porcine xenocorneal stroma obtained by decellularization is accompanied by a less intense increase in proteolysis factors and normalization of the protease/protease inhibitor ratio, as indicated by a significantly lower increase in the proteolysis index in the initial stages of the pathological percentage and its normalization by the end of the experiment.

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Publication of the article:

«Bulletin of problems biology and medicine», 2023 Issue 3, 170, 339-348 pages, index UDC 616.008.9-097.3:617.713-001.31-085.36]-092

DOI:

10.29254/2077-4214-2023-3-170-339-348