Sidashenko O. I., Voronkova O. S., Vinnikov A. I.

ACTIVITY OF LACTOBACILLI AGAINST BIOFILM-FORMING STRAINS OF S. EPIDERMIDIS

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Sidashenko O. I., Voronkova O. S., Vinnikov A. I.

Heading:

MICROBIOLOGY

Type of article:

Scientific article

Annotation:

Today, a major problem is the spread of diseases associated with the formation of infectious pathological biofilms. The microorganisms in the biofilm are highly resistant to antibiotics, which makes it difficult to treat such conditions. Staphylococci, especially S. epidermidis, which is part of the human microbiome, are often the cause of biofilm infections. Lactobacilli are representatives of normal microflora and perform a number of important functions in maintaining and preserving human health. This study investigated the antagonistic properties and the effect of the supernatant of lactobacillі on biofilm-forming strains of S. epidermidis. The study showed that strain of Lactobacillus 1 was established the highest antagonistic activity against all studied staphylococcal cultures — the largest growth inhibition zone was 18.2±0.6 mm. It was found that the supernatants obtained after cultivation strain of Lactobacillus 1 caused the highest level of decrease in the number of CFU/ ml in the studied staphylococcal cultures. It was revealed that the concentration cells strain of S. epidermidis 1 was decrease in 6.0×103 times, the content of staphylococci cells of strain 2 decreased by 4.7×103 times, and for strains 3 and 4 — by 3.7×103 and 6.6×103 times respectively, compared to the control. Thus, it can be concluded that it is promising to search for new strains of Lactobacillus isolated from various natural sources in order to obtain probiotic drugs that have preventive and therapeutic value against pathogens of biofilm infectious diseases.

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Bibliography:

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Publication of the article:

«Bulletin of problems biology and medicine», 2025 Issue 1, 176, 400-404 pages, index UDC 579.864.017:579.26

DOI:

10.29254/2077-4214-2025-1-176-400-404