Kolosova I. I.

THE INFLUENCE OF CADMIUM CHLORIDE ON THE HISTOSTRUCTURE OF THE OVARIES OF PREGNANT AND NON- PREGNANT RATS

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About the author:

Kolosova I. I.

Heading:

MORPHOLOGY

Type of article:

Scientific article

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Cadmium compounds have a negative impact on reproductive health and can lead to the formation of diseases during the prenatal period. The problems of female infertility require in-depth knowledge of the structure, blood supply and functioning of the ovaries both in normal conditions and under the influence of toxicants. Our study aimed to experimentally determine the potential gonadotoxic effect of cadmium chloride (at a dose of 1.0 mg/kg). For the study, 40 female Wistar rats were used, divided into 2 groups (control and experimental) depending on the solutions received. In each group, females were divided in half: pregnant and non-pregnant. The test substances were administered to the females intragastrically through a gavage tube once a day, at the same time throughout pregnancy, and on day 13, the animals were surgically killed, and the body was dissected, removing the ovaries for histological examination. In the control and experimental groups, the ovaries of female rats had a structure typical of other mammals, but in the experimental group exposed to cadmium chloride, changes in the histological structure of the ovaries of pregnant and non-pregnant females were found, namely thickening of the protein membrane, decreased follicle growth, increased number of antral and atretic follicles, irregular follicle borders, reduced, damaged, fragmented/absent ovocytes, degeneration of follicular granulosa cells; disorganisation, swelling and decreased number of corpus luteum, as well as increased number of fibrils in the connective tissue, hyperaemia of capillaries and venous vessels, thinning of blood vessel walls, and edema.

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Bibliography:

1. Pyrohova VI, Tsʹolko OR. Vyvchennya sotsialʹno-medychnykh aspektiv reproduktyvnoho zdorovʺya pidlitkiv. Reproduktyvne zdorovʺya zhinky. 2020;2(2):40-4. [in Ukrainian].
2. Kabinet Ministriv Ukrayiny. Rozporyadzhennya № 244-r. Pro skhvalennya Kontseptsiyi Derzhavnoyi prohramy «Reproduktyvne zdorov’ya natsiyi na 2006-2015 roky». Kyyiv: Kabinet Ministriv Ukrayiny; 2006 Kvit 27. Dostupno: https://zakon.rada.gov.ua/laws/show/244-2006- %D1%80#Text. [in Ukrainian].
3. Genchi G, Sinicropi MS, Lauria G, Carocci A, Catalano A. The Effects of Cadmium Toxicity. International J of Environmental Research and Public Health. 2020;17:3782. DOI: 10.3390/ijerph17113782.
4. Repić A, Bulat P, Antonijević B, Antunović M, Džudović J, Buha A, et al. The influence of smoking habits on cadmium and lead blood levels in the Serbian adult people. Environ. Sci. Pollut. Res. Int. 2020;27:751-60.
5. Rafati Rahimzadeh M, Rafati Rahimzadeh M, Kazemi S, Moghadamnia AA. Cadmium toxicity and treatment: An update. Caspian J Intern Med. 2017;8(3):135-45.
6. Lee S, Min J, Min K. Female infertility associated with blood lead and cadmium levels . International journal of environmental research and public health. 2020;17(5):1794. DOI: 10.3390/ijerph17051794.
7. Canipari R, De Santis L, Cecconi S. Female Fertility and Environmental Pollution. Int J Environ Res Public Health. 2020;17(23):8802. DOI: https://doi.org/10.3390/ijerph17238802.
8. World Health Organization. The public health impact of chemicals: knowns and unknowns. Geneva: WHO; Available from: https://www. who.int/ipcs/ assessment/public_health/chemicals_phc/en/.
9. Hryhoryuk IP, Kovbasenko RV, Dmytriyev OP, Teslyuk VV, Kovbasenko VM. Biolohichni osoblyvosti diyi kadmiyu na ekolohichnyy stan fitotsenoziv. Kyyiv: TSP «Komprynt»; 2019. 329 s. [in Ukrainian].
10. Sukhodolʹsʹka NV. Vmist tsynku, midi, svyntsyu ta kadmiyu v systemi maty - platsenta – plid. Eksperymentalʹna ta klinichna fiziolohiya i biokhimiya. 2015;2:69-77. [in Ukrainian].
11. Lamas GA, Navas-Acien A, Mark DB, Lee KL. Heavy metals, cardiovascular disease, and the unexpected benefits of edetate chelation therapy. J Am Coll. Cardiol. 2016;67:2411-8.
12. Parizi MG, Sedaghat Z, Mazloomi M, Fararouei M. Serum level of lead and cadmium is linked to facial cosmetics use among Iranian young women. Environ. Sci.Pollut. Res. Int. 2021;28:13913-8.
13. Sun J, Fang R, Wang H, Xu DX, Yang J, Huang X, et al. A review of environmental metabolism disrupting chemicals and effect biomarkers associating disease risks: where exposomics meets metabolomics. Environ. Int. 2022;158:106941. DOI: 10.1016/j.envint.2021.106941.
14. Rehman K, Fatima F, Waheed I, Akash MSH. Prevalence of exposure of heavy metals and their impact on health consequences. J. Cell Biochem. 2018;119:157-84.
15. de Angelis C, Galdiero M, Pivonello C, Salzano C, Gianfrilli D, Piscitelli P, et al. The environment and male reproduction: The effect of cadmium exposure on reproductive function and its implication in fertility. Reprod Toxicol. 2017;73:105-27.
16. Liu H, Wang R, OuYang H, Wang Y, Wu J, Li M, et al. Cadmium induced mouse spermatogonia apoptosis via mitochondrial calcium overload mediated by IP3R-MCU signal pathway. Toxicology. 2023;486:153448. Doi: https://doi.org/10.1016/j.tox.2023.153448.
17. Massányi M, Satarug S, Madeddu R, Stawarz R, Massányi P. Evidence for Ovarian and Testicular Toxicities of Cadmium and Detoxification by Natural Substances. Stresses. 2022;2(1):1-16.
18. Zhan S, Huang J. Effects of Cigarette Smoking on Preimplantation Embryo Development. Adv Exp Med Biol. 2021;1300:137-50.
19. Gull A, Dar A, Sharma M. Effects of Heavy Metals on the Health of Pregnant Women and Fetus: A Review. International J of Theoretical, Applied Sciences. 2018;10(1):1-9.
20. Nasiadek M, Danilewicz M, Sitarek, K, Świątkowska E, Daragó A, Stragierowicz J, et al. The effect of repeated cadmium oral exposure on the level of sex hormones, estrous cyclicity, and endometrium morphometry in female rats. Environ. Sci. Pollut. Res. 2018;25:28025-38.
21. Cheng Y, Zhang J, Wu T, Jiang X, Jia H, Qing S, et al. Reproductive toxicity of acute Cd exposure in mouse: Resulting in oocyte defects and decreased female fertility. Toxicol Appl Pharmacol. 2019 Sep 15;379:114684. DOI: 10.1016/j.taap.2019.114684.
22. Dong F, Li J, Lei WL, Wang F, Wang Y, Ouyang YC, et al. Chronic cadmium exposure causes oocyte meiotic arrest by disrupting spindle assembly 25 checkpoint and maturation promoting factor. Reprod Toxicol. 2020 Sep;96:141-9.
23. Massányi M, Satarug S, Madeddu R, Stawarz R, Massányi P. Evidence for Ovarian and Testicular Toxicities of Cadmium and Detoxification by Natural Substances. Stresses. 2022;2(1):1-16.
24. Li Z, Li T, Leng Y, Chen S, Liu Q, Feng J, et al. Hormonal changes and folliculogenesis in female offspring of rats exposed tocadmium during gestation and lactation. Environ. Pollut. 2018;238:336-47.
25. Massanyi P, Lukac N, Uhrín V, Toman R, Pivko J, Rafay J, et al. Female reproductive toxicology of cadmium. Acta Biol. Hung. 2007;58:287- 99.
26. Nasiadek M, Danilewicz M, Klimczak M, Stragierowicz J, Kilanowicz A. Subchronic Exposure to Cadmium Causes Persistent Changes in the Reproductive System in Female Wistar Rats. Oxidative Med. 2019 Dec 17:2019:6490820. DOI: https://doi.org/10.1155/2019/6490820.

Publication of the article:

«Bulletin of problems biology and medicine», 2024 Issue 1, 172, 401-411 pages, index UDC 612.3:591.39:661.852:661.782-092.9

DOI:

10.29254/2077-4214-2024-1-172-401-411